Proteomics analysis of high lipid-producing strain Mucor circinelloides WJ11: an explanation for the mechanism of lipid accumulation at the proteomic level

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• 作者：Xin Tang ; Xinyi Zan ; Lina Zhao ; Haiqin Chen ; Yong Q. Chen…
• 关键词：Lipid accumulation ; Mucor circinelloides ; Nitrogen deficiency ; Proteomics
• 刊名：Microbial Cell Factories
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：15
• 期：1
• 全文大小：1,976 KB
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• 作者单位：Xin Tang (1)
  Xinyi Zan (1)
  Lina Zhao (1)
  Haiqin Chen (1) (3)
  Yong Q. Chen (1) (3)
  Wei Chen (1) (3)
  Yuanda Song (1) (2)
  Colin Ratledge (4)
  
  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China
  3. Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, People’s Republic of China
  2. Colin Ratledge Center for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, People’s Republic of China
  4. Department of Biological Sciences, University of Hull, Hull, UK
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Applied Microbiology
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1475-2859

文摘

Background The oleaginous fungus, Mucor circinelloides, is attracting considerable interest as it produces oil rich in γ-linolenic acid. Nitrogen (N) deficiency is a common strategy to trigger the lipid accumulation in oleaginous microorganisms. Although a simple pathway from N depletion in the medium to lipid accumulation has been elucidated at the enzymatic level, global changes at protein levels upon N depletion have not been investigated. In this study, we have systematically analyzed the changes at the levels of protein expression in M. circinelloides WJ11, a high lipid-producing strain (36 %, lipid/cell dry weight), during lipid accumulation.