Aluminum Chloride Induces Osteoblasts Apoptosis via Disrupting Calcium Homeostasis and Activating Ca2+/CaMKII Signal Pathway
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- 作者：Zheng Cao ; Dawei Liu ; Qiuyue Zhang ; Xudong Sun…
- 关键词：AlCl3 ; Osteoblast apoptosis ; BAPTA ; AM ; Calcium homeostasis ; Ca2+/CaMKII signal pathway
- 刊名：Biological Trace Element Research
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：169
- 期：2
- 页码：247-253
- 全文大小：4,233 KB
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Dawei Liu (2) (3)
Qiuyue Zhang (1)
Xudong Sun (1)
Yanfei Li (1)
1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China
2. Heilongjiang Province Hospital, Harbin, 150036, China
3. School Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin, 150040, China - 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
- 出版者：Springer US
- ISSN：1559-0720
文摘
Aluminum promotes osteoblast (OB) apoptosis. Apoptosis is induced by the disordered calcium homeostasis. Therefore, to investigate the relationship between Al-induced OB apoptosis and calcium homeostasis, calvarium OBs from neonatal rats (3–4 days) were cultured and exposed to 0.048-mg/mL Al3+ or 0.048-mg/mL Al3+ combined with 5 μM BAPTA-AM (OBs were pretreated with 5 μM BAPTA-AM for 1 h, then added 0.048 mg/mL Al3+), respectively. Then OB apoptosis rate, intracellular calcium ions concentration ([Ca2+]i), mRNA expression level of calmodulin (CaM), and protein expression levels of CaM and p-CaMKII in OBs were examined. The result showed that AlCl3 increased OB apoptosis rate, and [Ca2+]i and p-CaMKII expression levels and decreased CaM expression levels, whereas BAPTA-AM relieved the effects. These results proved that AlCl3 induced OB apoptosis by disrupting the intracellular Ca2+ homeostasis and activating the Ca2+/CaMKII signal pathway. Our findings can provide new insights for revealing the apoptosis mechanism of OBs exposed to AlCl3. Keyword AlCl3 Osteoblast apoptosis BAPTA-AM Calcium homeostasis Ca2+/CaMKII signal pathway