Identification and fine mapping of qRBSDV-6 MH , a major QTL for resistance to rice black-streaked dwarf virus disease

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• 作者：Aihong Li (1)
  Cunhong Pan (1)
  Linbo Wu (2)
  Zhengyuan Dai (1)
  Shimin Zuo (2)
  Nin Xiao (1)
  Ling Yu (1)
  Yuhong Li (1)
  Xiaoxiang Zhang (1)
  Wenxia Xue (2)
  Hongxi Zhang (1)
  Xuebiao Pan (2)
  
• 关键词：qRBSDV ; 6 MH ; Rice black ; streaked dwarf virus disease ; Major resistance QTL ; Fine mapping
• 刊名：Molecular Breeding
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：32
• 期：1
• 页码：1-13
• 全文大小：579KB
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• 作者单位：Aihong Li (1)
  Cunhong Pan (1)
  Linbo Wu (2)
  Zhengyuan Dai (1)
  Shimin Zuo (2)
  Nin Xiao (1)
  Ling Yu (1)
  Yuhong Li (1)
  Xiaoxiang Zhang (1)
  Wenxia Xue (2)
  Hongxi Zhang (1)
  Xuebiao Pan (2)
  
  1. Lixiahe Agricultural Research Institute, Yangzhou, 225007, Jiangsu Province, People’s Republic of China
  2. Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, 225009, People’s Republic of China
  
• ISSN：1572-9788

文摘

Rice black streaked-dwarf virus (RBSDV) disease is recently expanding in southern China and poses a serious threat to rice crops. Few studies related to the genetics and breeding of RBSDV resistance have been reported. We have previously mapped a number of quantitative trait loci (QTLs) for RBSDV resistance by using a recombinant inbred line population of ‘Zhenshan 97-(ZS97, susceptible)/‘Minghui 63-(MH63, resistant) with natural infection data in two locations. In the present study, we confirmed the presence of a number of resistant QTLs on chromosomes 6, 7, and 9 from MH63 by using the same population in four different locations. We then focused on a major QTL, qRBSDV-6 MH , on chromosome 6 and introduced it into a highly susceptible japonica rice variety, ‘Huaidao 5- using MH63 as the donor via marker-assisted selection, to generate seven backcross inbred lines (BILs). Natural infection and artificial inoculation-based tests revealed that all of the BILs had a significantly higher resistance to RBSDV than the recurrent parent. These results demonstrate that qRBSDV-6 MH is a stable major resistance QTL of high breeding value. We also constructed a set of chromosome segment substitution lines (CSSLs) specific to the qRBSDV-6 MH region and these used as fine mapping population. Combining the genotypes of CSSLs with the phenotypes from natural infection data in a highly RBSDV epidemic area during two different sowing seasons, we were able to precisely map qRBSDV-6 MH to the markers S18 and S23 at a physical distance of 627.6?kb on the Nipponbare reference genome.