Biodegradation of Enteromorpha polysaccharides by intestinal micro-community from Siganus oramin

详细信息    查看全文

• 作者：Zhibiao Zhang ; Xuefeng Han ; Yan Xu ; Jin Li…
• 关键词：micro ; community ; enteromorpha polysaccharide ; biodegradation ; denaturing gradient gel electrophoresis ; 16S rRNA gene clone library
• 刊名：Journal of Ocean University of China
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：15
• 期：6
• 页码：1034-1038
• 全文大小：359 KB
• 刊物主题：Oceanography; Meteorology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5021
• 卷排序：15

文摘

Micro-communities are supposed to have more potential functions of biodegradation of polysaccharides than single strain; however, the intestinal micro-communities involved in the biodegradation of Enteromorpha polysaccharides (EP) were seldom reported. In order to obtain the EP-degrading micro-community, the intestines of Siganus oramin was obtained to isolate the micro-communities, which were enriched by 0.3% of EP as the sole carbon source. A stable micro-community with EP degradative capability was achieved after seven generations of subculture, named H1. Results showed that H1 was able to degrade 75% of EP within 24 hours, and the activity of EP lyases reached 500 U mL−1 in 32 hours. With denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis, ten bacteria closely related to Marinomonas pontica, Microbacterium sp., Leucobacter chironomi, Cyclobacterium sp., Algoriphagus winogradskyi, Pseudoalteromonas sp. and Vibrio sp. were determined. Furthermore, compared with the DGGE bands sequence and the clone library analysis, the dominant bacteria of the EP-biodegrading micro- community were Pseudoalteromonas sp. and Vibrio sp., with the respective proportion of 38% and 46%, and they should play an important role in EP degradation together with other degrading bacteria in the micro-community H1.