Recombinant salmonella-based 4-1BBL vaccine enhances T cell immunity and inhibits the development of colorectal cancer in rats: in vivo effects of vaccine containing 4-1BBL

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• 作者：Jianxin Ye (1)
  Ling Li (1)
  Yuanting Zhang (1)
  Xueguang Zhang (2)
  Daming Ren (3)
  Weichang Chen (1)
  
• 关键词：Recombinant attenuated salmonella ; Colorectal cancer ; 4 ; 1BBL ; T cell ; based cellular immunity
• 刊名：Journal of Biomedical Science
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：20
• 期：1
• 全文大小：2,800 KB
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• 作者单位：Jianxin Ye (1)
  Ling Li (1)
  Yuanting Zhang (1)
  Xueguang Zhang (2)
  Daming Ren (3)
  Weichang Chen (1)
  
  1. Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, 215006, China
  2. Institute of Medical Biotechnology, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Soochow University, 215007, Suzhou, China
  3. Institute of Genetics, State Key Laboratory of Genetic Engineering of Fudan University, 200433, Shanghai, China
  
• ISSN：1423-0127

文摘

Background Immunotherapy with vaccines is attractive for the treatment of cancer. This study is aimed at determining the effect of recombinant Salmonella (SL3261)-based 4-1BB ligand (4-1BBL) vaccine on the development of colorectal cancers and the potential immune mechanisms in rats. Results In comparison with that in the PBS group, similar levels of 4-1BBL expression, the frequency of T cells, IFN-γ responses, and comparable numbers of tumors were detected in the SL3261 and SL3261C groups of rats. In contrast, significantly fewer numbers of tumors, increased levels of 4-1BBL expression in the spleens and colorectal tissues, higher frequency of peripheral blood and splenic CD3+CD25+ T cells, and stronger splenic T cell IFN-γ responses were detected in the SL3261R group of rats. Conclusion Our results indicated that vaccination with recombinant attenuated Salmonella harboring the 4-1BBL gene efficiently enhanced T cell immunity and inhibited the development of carcinogen-induced colorectal cancers in rats.