RpoS-Dependent Expression of OsmY in Salmonella enterica serovar Typhi: Activation Under Stationary Phase and SPI-2-Inducing Conditions

详细信息    查看全文

• 作者：Xueming Zheng ; Ying Ji ; Xiaoqin Weng ; Xinxiang Huang
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：70
• 期：6
• 页码：877-882
• 全文大小：928 KB
• 参考文献：1.Cameron AD, Dorman CJ (2012) A fundamental regulatory mechanism operating through OmpR and DNA topology controls expression of Salmonella pathogenicity islands SPI-1 and SPI-2. PLoS Genet 8:e1002615View Article PubMed Central PubMed
  2.Coombes BK, Brown NF, Valdez Y, Brumell JH, Finlay BB (2004) Expression and secretion of Salmonella pathogenicity island-2 virulence genes in response to acidification exhibit differential requirements of a functional type III secretion apparatus and SsaL. J Biol Chem 279:49804-9815View Article PubMed
  3.Crump JA, Mintz ED (2010) Global trends in typhoid and paratyphoid fever. Clin Infect Dis 50:241-46View Article PubMed Central PubMed
  4.de Jong HK, Parry CM, van der Poll T, Wiersinga WJ (2012) Host-pathogen interaction in invasive Salmonellosis. PLoS Pathog 8:e1002933View Article PubMed Central PubMed
  5.Dong T, Schellhorn HE (2010) Role of RpoS in virulence of pathogens. Infect Immun 78:887-97View Article PubMed Central PubMed
  6.Du H, Sheng X, Zhang H, Zou X, Ni B, Xu S, Zhu X, Xu H, Huang X (2011) RpoE may promote flagellar gene expression in Salmonella enterica serovar Typhi under hyperosmotic stress. Curr Microbiol 62:492-00View Article PubMed
  7.Du H, Wang M, Luo Z, Ni B, Wang F, Meng Y, Xu S, Huang X (2011) Coregulation of gene expression by sigma factors RpoE and RpoS in Salmonella enterica serovar Typhi during hyperosmotic stress. Curr Microbiol 62:1483-489View Article PubMed
  8.Ellermeier JR, Slauch JM (2007) Adaptation to the host environment: regulation of the SPI1 type III secretion system in Salmonella enterica serovar Typhimurium. Curr Opin Microbiol 10:24-9View Article PubMed
  9.Feklistov A, Sharon BD, Darst SA, Gross CA (2014) Bacterial sigma factors: a historical, structural, and genomic perspective. Annu Rev Microbiol 68:357-76View Article PubMed
  10.Figueira R, Holden DW (2012) Functions of the Salmonella pathogenicity island 2 (SPI-2) type III secretion system effectors. Microbiology 158:1147-161View Article PubMed
  11.Gong H, Su J, Bai Y, Miao L, Kim K, Yang Y, Liu F, Lu S (2009) Characterization of the expression of Salmonella type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice. BMC Microbiol 9:73View Article PubMed Central PubMed
  12.Jishage M, Ishihama A (1995) Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of sigma 70 and sigma 38. J Bacteriol 177:6832-835PubMed Central PubMed
  13.Jishage M, Iwata A, Ueda S, Ishihama A (1996) Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of four species of sigma subunit under various growth conditions. J Bacteriol 178:5447-451PubMed Central PubMed
  14.Lange R, Hengge-Aronis R (1994) The cellular concentration of the sigma S subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability. Genes Dev 8:1600-612View Article PubMed
  15.Lawhon SD, Khare S, Rossetti CA, Everts RE, Galindo CL, Luciano SA, Figueiredo JF, Nunes JE, Gull T, Davidson GS, Drake KL, Garner HR, Lewin HA, Baumler AJ, Adams LG (2011) Role of SPI-1 secreted effectors in acute bovine response to Salmonella enterica serovar Typhimurium: a systems biology analysis approach. PLoS One 6:e26869View Article PubMed Central PubMed
  16.Liechty A, Chen J, Jain MK (2000) Origin of antibacterial stasis by polymyxin B in Escherichia coli. Biochim Biophys Acta 1463:55-4View Article PubMed
  17.McGhie EJ, Brawn LC, Hume PJ, Humphreys D, Koronakis V (2009) Salmonella takes control: effector-driven manipulation of the host. Curr Opin Microbiol 12:117-24View Article PubMed Central PubMed
  18.Nikolaus T, Deiwick J, Rappl C, Freeman JA, Schroder W, Miller SI, Hensel M (2001) SseBCD proteins are secreted by the type III secretion system of Salmonella pathogenicity island 2 and function as a translocon. J Bacteriol 183:6036-045View Article PubMed Central PubMed
  19.Oh JT, Cajal Y, Skowronska EM, Belkin S, Chen J, Van Dyk TK, Sasser M, Jain MK (2000) Cationic peptide antimicrobials induce selective transcription of micF and osmY in Escherichia coli. Biochim Biophys Acta 1463:43-4View Article PubMed
  20.Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2006) Conserved and variable functions of the sigmaE stress response in related genomes. PLoS Biol 4:e2View Article PubMed Central PubMed
  21.Srikanth CV, Mercado-Lubo R, Hallstrom K, McCormick BA (2011) Salmonella effector proteins and host-cell responses. Cell Mol Life Sci 68:3687-697View Article PubMed Central PubMed
  22.Xu S, Zhang H, Sheng X, Xu H, Huang X (2008) Transcriptional expression of fljB: z66, a flagellin gene located on a novel linear plasmid of Salmonella enterica serovar Typhi under environmental stresses. New Microbiol 31:241-47PubMed
  23.Yeats C, Bateman A (2003) The BON domain: a putative membrane-binding domain. Trends Bio
• 作者单位：Xueming Zheng (1)
  Ying Ji (1)
  Xiaoqin Weng (1)
  Xinxiang Huang (1)
  
  1. Department of Biochemistry and Molecular Biology, School of Medicine, Jiangsu University, Zhenjiang, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

OsmY is a periplasmic protein with two BON domains which may attach to phospholipid membranes. Previous reports showed that the expression of OsmY in Escherichia coli was hyperosmotically inducible and RpoS dependent. But little work was done to investigate the expression and function of OsmY in Salmonella. Here, we detected the endogenous OsmY in Salmonella enterica serovar Typhi (S. Typhi) with polyclonal antibody. The results showed that the expression of OsmY was also RpoS dependent and was activated under stationary phase. Further, using in vitro culture, we established the Salmonella pathogenesis island (SPI)-1 and SPI-2-inducing conditions with hyperosmolarity and low-phosphate, low-magnesium medium (pH 5.8), respectively, and found that only SPI-2-inducing conditions can activate the expression of OsmY. osmY deletion mutant showed delayed growth compared with wild-type S. Typhi in SPI-2-inducing conditions. The results indicated that OsmY may function to resist the stress and be favorable for Salmonella’s replication in the Salmonella-containing vesicles of macrophage.