Identification of superoxide dismutase isoenzymes in tobacco pollen

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• 作者：Jing Wang (1)
  Xuequn Liu (1)
  Guanghui Yu (1)
  
• 关键词：tobacco ; pollen ; reactive oxygen species ; superoxide dismutase ; fertilization
• 刊名：Frontiers in Biology
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：4
• 期：4
• 页码：442-445
• 全文大小：108KB
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• 作者单位：Jing Wang (1)
  Xuequn Liu (1)
  Guanghui Yu (1)
  
  1. Key Laboratory for Biotechnology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan, 430074, China
  
• ISSN：1674-7992

文摘

We investigated the possible existence of superoxide dismutase (SOD; EC 1.15.1.1) isoenzymes in the pollen of Nicotiana tabacum (Petit Havana SR-1 cultivar). To detect SOD activity, crude extracts from tobacco pollen were subjected to native polyacrylamide gel electrophoresis followed by staining with nitroblue tetrazolium (NBT). The presence of six SOD isoenzymes was detected in tobacco pollen. Treatment with SOD inhibitors indicated the presence of one manganese SOD (Mn SOD), five copper-zinc SOD (Cu/Zn SOD) isoenzymes, and the absence of iron SOD (Fe SOD).