Biomimetic Enzyme-linked Immunosorbent Assay Using a Hydrophilic Molecularly Imprinted Membrane for Recognition and Fast Determination of Trichlorfon and Acephate Residues in Vegetables

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• 作者：Chen Shi ; Xueyan Liu ; Lingyu Song ; Xuguang Qiao ; Zhixiang Xu
• 关键词：Molecular imprinting ; Hydrophilic imprinted membrane ; Biomimetic antibody ; Enzyme ; linked immunosorbent assay ; Multi ; pesticide residues ; Asparagus ; Cucumber
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：8
• 期：10
• 页码：2496-2503
• 全文大小：719 KB
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• 作者单位：Chen Shi (1)
  Xueyan Liu (1)
  Lingyu Song (1)
  Xuguang Qiao (1)
  Zhixiang Xu (1)
  
  1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

This study describes the development of a new direct competitive enzyme-linked immunosorbent assay method with a hydrophilic imprinted film as biomimetic antibody for determining multi-pesticide residues. The imprinted film, which can selectively recognize trichlorfon and acephate, was synthesized directly on the surface of a 96-well plate using 4-(dimethoxyphosphorothioylamino) butanoic acid as the template molecule. This film exhibited antibody-like recognition ability, fast adsorption–desorption dynamics and good stability. Under optimal conditions, the sensitivity (IC₅₀) of the biomimetic enzyme-linked immunosorbent assay (BELISA) method was 12.0 mg/L for trichlorfon and 30.0 mg/L for acephate. The limit of detection (LOD, IC₁₅) was 8.0 μg/L for trichlorfon and 12.0 μg/L for acephate. The developed method was applied to the determination of the trichlorfon and acephate in the spiked asparagus and cucumber samples with recoveries ranging from 72.1 to 92.0 % for trichlorfon and 70.0 to 85.0 % for acephate. Keywords Molecular imprinting Hydrophilic imprinted membrane Biomimetic antibody Enzyme-linked immunosorbent assay Multi-pesticide residues Asparagus Cucumber