Quantifiable fluorescent glycan microarrays

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• 作者：Xuezheng Song ; Baoyun Xia ; Yi Lasanajak ; David F. Smith and Richard D. Cummings
• 关键词：Carbohydrate ; Glycan conjugate ; Glycomics ; Microarray ; Fluorescence
• 刊名：Glycoconjugate Journal
• 出版年：2008
• 出版时间：January, 2008
• 年：2008
• 卷：25
• 期：1
• 页码：15-25
• 全文大小：715.0 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-4986

文摘

A glycan microarray was developed by using 2,6-diaminopyridine (DAP) as a fluorescent linker and printing of the glycan-DAP conjugates (GDAPs) on epoxy-activated glass slides. Importantly, all coupled GDAPs showed a detectable level of concentration-dependent GDAP fluorescence under blue laser excitation (495 nm) that can be used for both grid location and on-slide quantification. A glycan array including a large number of GDAP’s derived from natural and commercially available free glycans was constructed and glycan interactions with various plant lectins were investigated. In addition, binding parameters of lectins to glycans were obtained by varying both the amount of GDAPs on the array and the lectin concentration in analyses. These data demonstrate the general utility of GDAP microarrays for functional glycomic analyses and for determining binding parameters of glycan binding proteins (GBPs).