Development of the CAS-LIBB single-particle microbeam for localized irradiation of living cells

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• 作者：Xufei Wang (1) (2)
  Xiaohua Wang (1) (2)
  Lianyun Chen (1) (2)
  Zhiwen Hu (1) (2)
  Jun Li (1) (2)
  Yu Wu (1) (2)
  Bin Chen (1) (2)
  Suhua Hu (1) (2)
  Jun Zhang (1) (2)
  Mingliang Xu (1) (2)
  Lijun Wu (1) (2)
  Shaohu Wang (1) (2)
  Huiyun Feng (1) (2)
  Furu Zhan (1) (2)
  Shixiang Peng (1) (2)
  Chundong Hu (1) (2)
  Shuqing Zhang (1) (2)
  Jianjun Cheng (1) (2)
  Zhongtao Shi (1) (2)
  Hang Yuan (1) (2)
  Haitao Yuan (1) (2)
  Zengliang Yu (1) (2)
  
• 关键词：microbeam facility ; single ; particle irradiation ; particle detection precision ; target location accuracy ; living cells
• 刊名：Chinese Science Bulletin
• 出版年：2004
• 出版时间：September 2004
• 年：2004
• 卷：49
• 期：17
• 页码：1806-1811
• 全文大小：1124KB
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• 作者单位：Xufei Wang (1) (2)
  Xiaohua Wang (1) (2)
  Lianyun Chen (1) (2)
  Zhiwen Hu (1) (2)
  Jun Li (1) (2)
  Yu Wu (1) (2)
  Bin Chen (1) (2)
  Suhua Hu (1) (2)
  Jun Zhang (1) (2)
  Mingliang Xu (1) (2)
  Lijun Wu (1) (2)
  Shaohu Wang (1) (2)
  Huiyun Feng (1) (2)
  Furu Zhan (1) (2)
  Shixiang Peng (1) (2)
  Chundong Hu (1) (2)
  Shuqing Zhang (1) (2)
  Jianjun Cheng (1) (2)
  Zhongtao Shi (1) (2)
  Hang Yuan (1) (2)
  Haitao Yuan (1) (2)
  Zengliang Yu (1) (2)
  
  1. Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, 230031, Hefei, China
  2. Key Laboratory of Ion Beam Bioengineering in Anhui Province, Chinese Academy of Sciences, 230031, Hefei, China
  
• ISSN：1861-9541

文摘

A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydrogen ions, produced by a van de Graaff accelerator, in an energy range of 2.0-.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation protocols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.