Complete genome sequence of methicillin-sensitive Staphylococcus aureus containing a heterogeneic staphylococcal cassette chromosome element

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• 作者：DeZhi Li (14453) (24453)
  YaNan Chu (34453) (44453)
  LuFeng Ren (34453) (44453)
  XinGang Li (34453) (44453)
  Lina Yuan (34453) (44453)
  Yu Kang (34453)
  Wei Zhang (54453)
  Yu Yang (64453)
  XuMin Wang (34453)
  J. Kenneth Baillie (74453) (84453)
  Jun Yu (34453) (44453)
  ZhanCheng Gao (14453)
  
• 关键词：Staphylococcus aureus ; staphylococcal cassette chromosome mec (SCC ; mec) ; genomics ; pyrosequencing
• 刊名：Science China Life Sciences
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：56
• 期：3
• 页码：268-274
• 全文大小：601KB
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• 作者单位：DeZhi Li (14453) (24453)
  YaNan Chu (34453) (44453)
  LuFeng Ren (34453) (44453)
  XinGang Li (34453) (44453)
  Lina Yuan (34453) (44453)
  Yu Kang (34453)
  Wei Zhang (54453)
  Yu Yang (64453)
  XuMin Wang (34453)
  J. Kenneth Baillie (74453) (84453)
  Jun Yu (34453) (44453)
  ZhanCheng Gao (14453)
  
  14453. Department of Respiratory and Critical Care Medicine, Peking University People鈥檚 Hospital, Beijing, 100044, China
  24453. Department of Respiratory Medicine, Shandong Provincial Hospital, Shandong University, Jinan, 250021, China
  34453. CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China
  44453. The DNA Sequencing Technologies R&D Center, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China
  54453. Department of Respiratory Medicine, First Affiliated Hospital of Nanchang University, Nanchang, 330006, China
  64453. Chinese Academy of Inspection and Quarantine, Beijing, 100025, China
  74453. Division of Genetics and Genomics, The Roslin Institute, University of Edinburgh, Roslin, EH25 9RG, UK
  84453. Department of Critical Care Medicine, University of Edinburgh, Edinburgh, EH16 4TJ, UK
  

文摘

Staphylococcus aureus is a common human bacterium that sometimes becomes pathogenic, causing serious infections. A key feature of S. aureus is its ability to acquire resistance to antibiotics. The presence of the staphylococcal cassette chromosome (SCC) element in serotypes of S. aureus has been confirmed using multiplex PCR assays. The SCC element is the only vector known to carry the mecA gene, which encodes methicillin resistance in S. aureus infections. Here, we report the genome sequence of a novel methicillin-sensitive S. aureus (MSSA) strain: SCC-like MSSA463. This strain was originally erroneously serotyped as methicillin-resistant S. aureus in a clinical laboratory using multiplex PCR methods. We sequenced the genome of SCC-like MSSA463 using pyrosequencing techniques and compared it with known genome sequences of other S. aureus isolates. An open reading frame (CZ049; AB037671) was identified downstream of attL and attR inverted repeat sequences. Our results suggest that a lateral gene transfer occurred between S. aureus and other organisms, partially changing S. aureus infectivity. We propose that attL and attR inverted repeats in S. aureus serve as frequent insertion sites for exogenous genes.