Effects of Nano-Anatase on Ribulose-1, 5-Bisphosphate Carboxylase/Oxygenase mRNA Expression in Spinach
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- 作者：Wang Xuming (1) (2)
Gao Fengqing (1)
Ma Linglan (1)
Liu Jie (1)
Yin Sitao (1)
Yang Ping (3)
Hong Fashui (1) - 关键词：Nano ; anatase ; TiO2 ; Spinach ; Rubisco ; rbcL ; rbcS
- 刊名：Biological Trace Element Research
- 出版年：2008
- 出版时间：December 2008
- 年：2008
- 卷：126
- 期：1-3
- 页码：280-289
- 全文大小：339KB
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Gao Fengqing (1)
Ma Linglan (1)
Liu Jie (1)
Yin Sitao (1)
Yang Ping (3)
Hong Fashui (1)
1. Medical College of Suzhou University, Suzhou, 215123, People鈥檚 Republic of China
2. Department of Life Sciences, Huaibei Coal Industry Teachers College, Huaibei, 235000, People鈥檚 Republic of China
3. College of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 215123, People鈥檚 Republic of China
文摘
Being a proven photocatalyst, nano-anatase is capable of undergoing electron transfer reactions under light. In previous studies, we had proven that nano-anatase improved photosynthesis and greatly promoted spinach growth. However, the molecular mechanisms of carbon reaction promoted by nano-anatase are still not clearly understood. In the present paper, reverse transcription polymerase chain reaction and Northern blotting were carried out, and the results showed that the expression of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit (rbcS) and Rubisco large subunit (rbcL) messenger RNA (mRNA) in the nano-anatase-treated spinach were obviously promoted, respectively, while the effects of bulk TiO2 were not so significant. Accordingly, the protein expression of Rubisco from the nano-anatase-treated spinach was increased by 40% compared with control. Further analysis indicated that the activity of Rubisco in the nano-anatase-treated spinach was significantly higher than the control, by up to 2.33 times, and bulk TiO2 treatment had no such significant effects. Together, one of the molecular mechanisms of carbon reaction promoted by nano-anatase is that the nano-anatase treatment results in the enhancement of Rubisco mRNA amounts, the protein levels, and activity of Rubisco, thereby leading to the improvement of Rubisco carboxylation and the high rate of photosynthetic carbon reaction.