Quantitative comparison of the expression of myogenic regulatory factors in flounder (Paralichthys olivaceus) embryos and adult tissues
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- 作者：Yuqing Zhang (1) (2)
Xungang Tan (1)
Peng Xu (1) (2)
Wei Sun (1) (2)
Yongli Xu (1)
Peijun Zhang (1) - 关键词：Paralichthys olivaceus ; myogenic regulatory factors ; myogenesis ; muscle ; quantitative RT ; PCR
- 刊名：Chinese Journal of Oceanology and Limnology
- 出版年：2010
- 出版时间：March 2010
- 年：2010
- 卷：28
- 期：2
- 页码：248-253
- 全文大小：755KB
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Xungang Tan (1)
Peng Xu (1) (2)
Wei Sun (1) (2)
Yongli Xu (1)
Peijun Zhang (1)
1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
2. Graduate School of Chinese Academy of Sciences, Beijing, 100039, China - ISSN：1993-5005
文摘
MyoD, Myf5, and myogenin are myogenic regulatory factors that play important roles during myogenesis. It is thought that MyoD and Myf5 are required for myogenic determination, while myogenin is important for terminal differentiation and lineage maintenance. To better understand the function of myogenic regulatory factors in muscle development of flounder, an important economic fish in Asia, real-time quantitative RT-PCR was used to characterize the expression patterns of MyoD, Myf5, and myogenin at early stages of embryo development, and in different tissues of the adult flounder. The results show that, Myf5 is the first gene to be expressed during the early stages of flounder development, followed by MyoD and myogenin. The expressions of Myf5, yoD, and myogenin at the early stages have a common characteristic: expression gradually increased to a peak level, and then gradually decreased to an extremely low level. In the adult flounder, the expression of the three genes in muscle is much higher than that in other tissues, indicating that they are important for muscle growth and maintenance of grown fish. During embryonic stages, the expression level of MyoD might serve an important role in the balance between muscle cell differentiation and proliferation. When the MyoD expression is over 30% of its highest level, the muscle cells enter the differentiation stage.