Total Glucosides of Peony Attenuates 2,4,6-Trinitrobenzene Sulfonic Acid/Ethanol-Induced Colitis in Rats Through Adjustment of Th1/Th2 Cytokines Polarization

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• 作者：Yabing Zhang (1)
  Rui Zhou (2) (3)
  Feng Zhou (1)
  Hong Cheng (1)
  Bing Xia (1) (2) (3)
  
• 关键词：TNBS/ethanol ; induced colitis ; Total glucosides of peony (TGP) ; Tumor necrosis factor ; α (TNF ; α) ; Interleukin ; 1β (IL ; 1β) ; Interleukin ; 10 (IL ; 10)
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：68
• 期：1
• 页码：83-95
• 全文大小：973 KB
• 作者单位：Yabing Zhang (1)
  Rui Zhou (2) (3)
  Feng Zhou (1)
  Hong Cheng (1)
  Bing Xia (1) (2) (3)
  
  1. Department of Gastroenterology, Zhongnan Hospital of Wuhan University School of Medicine, Donghu Road 169, Wuhan, 430071, Hubei, People’s Republic of China
  2. Hubei Clinical Center for Intestinal & Colorectal Diseases, Wuhan, 430071, People’s Republic of China
  3. Hubei Key Laboratory for Intestinal & Colorectal Diseases, Wuhan, 430071, People’s Republic of China
  
• ISSN：1559-0283

文摘

The present study is to investigate effects of total glucosides of peony (TGP) on 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol-induced colitis in rats and to explore potential clinical use of TGP for treatment of inflammatory bowel disease. Sixty Sprague–Dawley rats were randomly grouped into normal controls, model controls, sulfasalazine (SASP) controls (100?mg/kg/day), and low, medium, and high-dose TGP groups (25, 50, and 100?mg/kg/day, respectively). 24?h following colonic instillation of TNBS, TGP, and SASP were given by gastric gavage three times a day for 7?days. Disease activity index (DAI), colon macroscopic damage index (CMDI), histopathological score (HPS), and myeloperoxidase (MPO) activity were evaluated. Levels of serum TNF-α, IL-1β, and IL-10 were measured by ELISA, and expression of TNF-α, IL-1β, and IL-10 mRNA and protein in colonic tissues was detected by RT-PCR and western blot, respectively. Compared with rats in the model controls, TGP (50 or 100?mg/kg/day)-treated rats with TNBS/ethanol-induced colitis showed significant improvements of DAI, CMDI, HPS, and MPO activity. Moreover, administration of TGP (50 or 100?mg/kg/day) decreased the up-regulated levels of serum TNF-α and IL-1β, and expression of TNF-α and IL-1β mRNA and protein in colonic tissues, and increased the serum IL-10 and colonic IL-10 mRNA and protein level. And there was no significant difference compared with administration of SASP (P?>?0.05). TGP attenuates TNBS/ethanol-induced colitis in rats and its efficacy is similar to SASP, the potential mechanism might be related to the adjustment of Th1/Th2 cytokines polarization by decreasing pro-inflammatory cytokine TNF-α and IL-1β, and increasing anti-inflammatory cytokine IL-10.