Molecular characterization and expression of the MYND-ZF gene from Clonorchis sinensis

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• 作者：Lexun Wang ; Xinbing Yu ; Yabo Yang ; Jingfang Chen ; Dong Hu ; Chuanhuan Deng ; Xiao Yang ; Xuchu Hu and Jin Xu
• 刊名：Parasitology Research
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：107
• 期：5
• 页码：1249-1255
• 全文大小：683.8 KB

文摘

The MYND-type zinc finger protein (MYND-ZF) is a large group of proteins containing the MYND domain which play an important role in protein–protein interactions. A cDNA clone encoding a novel MYND-ZF was isolated and identified from a Clonorchis sinensis (C. sinensis) adult cDNA library. The open reading frame of this novel cDNA sequence contains 1,440 base pairs with a putative protein of 479 amino acids showing a high homology with the MYND-ZF identified from other species. Recombinant CsMYND-ZF was expressed and purified from Escherichia coli BL21 (DE3). CsMYND-ZF transcripts were detected in the cDNA of adult worms and metacercariae but not in eggs of C. sinensis. Immunohistochemistry results revealed that CsMYND-ZF was deposited at the tegument of adult worms and metacercariae C. sinensis using anti-recombinant CsMYND-ZF serum. These findings may contribute to the development of a reliable diagnostic method.