Isolation and characterization of bacterial endophytes of Curcuma longa L.

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• 作者：Ajay Kumar ; Ritu Singh ; Akhilesh Yadav ; D. D. Giri ; P. K. Singh…
• 关键词：Antimicrobial activity ; Curcuma longa ; Endophyte ; 16S rRNA analysis ; Substrate utilization
• 刊名：3 Biotech
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：1
• 全文大小：461 KB
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• 作者单位：Ajay Kumar (1)
  Ritu Singh (1)
  Akhilesh Yadav (1)
  D. D. Giri (1)
  P. K. Singh (1)
  Kapil D. Pandey (1)
  
  1. Centre of Advance Study in Botany, Banaras Hindu University, Varanasi, 221005, India
  
• 刊物主题：Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-5738

文摘

Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp. (ECL3), Bacillus pumilis (ECL4), Pseudomonas putida (ECL5), and Clavibacter michiganensis (ECL6). All the strains produced IAA and solubilized phosphate and only two strains produced siderophore (ECL3 and ECL5) during plant growth promoting trait analysis. All the endophytic strains utilized glucose, sucrose and yeast extract as a carbon source where as glycine, alanine, cystine and glutamine as nitrogen source. The strains were mostly sensitive to antibiotic chloramphenicol followed by erythromycin while resistant to polymixin B. The endophytic strains effectively inhibit the growth of Escherichia coli, Klebsiella pneumoniae and some of the fungal strain like Fusarium solani and Alterneria alternata. The strain ECL2 and ECL4 tolerated maximum 8 % of NaCl concentration where as strains ECL5 and ECL6 6 % in salinity tolerance. Keywords Antimicrobial activity Curcuma longa Endophyte 16S rRNA analysis Substrate utilization