Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors

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• 作者：Jing Sun (1)
  Zhigang Yu (2)
  Yahui Gao (3)
  Qianqian Zhou (3)
  Yu Zhen (4)
  Hongtao Chen (2)
  Liyuan Zhao (1)
  Qingzhen Yao (2)
  Tiezhu Mi (4)
  
• 关键词：canonical correspondence analysis (CCA) ; denaturing gradient gel electrophoresis (DGGE) ; phytoplankton ; East China Sea ; Yellow Sea
• 刊名：Chinese Journal of Oceanology and Limnology
• 出版年：2010
• 出版时间：March 2010
• 年：2010
• 卷：28
• 期：2
• 页码：315-322
• 全文大小：661KB
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• 作者单位：Jing Sun (1)
  Zhigang Yu (2)
  Yahui Gao (3)
  Qianqian Zhou (3)
  Yu Zhen (4)
  Hongtao Chen (2)
  Liyuan Zhao (1)
  Qingzhen Yao (2)
  Tiezhu Mi (4)
  
  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China
  2. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China
  3. School of Life Sciences, Xiamen University, Xiamen, 361021, China
  4. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, 266100, China
  
• ISSN：1993-5005

文摘

Relationships between phytoplankton community composition and environmental variables in the East China Sea (ECS) and Yellow Sea (YS) were investigated using geochemical and molecular microbiology methods. The diversity of phytoplankton was characterized using cultivation-independent PCR-based denaturing gradient gel electrophoresis (DGGE). Groups resulting from unweighted pair-group method with arithmetic averages clustering of the DGGE profiles showed good consistency with the eco-environmental characteristics of the sea area they belonged to. Additionally, the clustering results based on DGGE fingerprinting and those based on morphological compositions were practically identical. The relationship of phytoplankton diversity to environmental factors was statistically analyzed. Temperature, dissolved inorganic nitrogen (DIN), and silicate-Si were found significantly related to the phytoplankton community composition. Canonical correspondence analysis (CCA) was performed to reveal the relationship between community composition and these three environmental factors. Generally, values of the ECS are clearly separated from those of the YS in the CCA biplot, due to mainly the effect of temperature and DIN.