The effect of fruit bagging on the color, phenolic compounds and expression of the anthocyanin biosynthetic and regulatory genes on the ?ranny Smith?apples

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• 作者：Lixin Wang (1)
  Xiaojun Zhang (1)
  Yulian Liu (1)
  Xingyun Shi (1)
  Yajie Wang (1)
  Chuanfeng Zhang (1)
  Zhengyang Zhao (1)
  
• 关键词：Apple ; Anthocyanins ; Phenolic compounds ; Bagging treatment ; Gene expression
• 刊名：European Food Research and Technology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：237
• 期：6
• 页码：875-885
• 全文大小：
• 作者单位：Lixin Wang (1)
  Xiaojun Zhang (1)
  Yulian Liu (1)
  Xingyun Shi (1)
  Yajie Wang (1)
  Chuanfeng Zhang (1)
  Zhengyang Zhao (1)
  
  1. College of Horticulture, Northwest A & F University, Yangling, 712100, Shaanxi, China
  
• ISSN：1438-2385

文摘

In the Loess Plateau region of China, it was discovered that the famous green-skinned apple cultivar ?ranny Smith?could rapidly turn red following bag removal. In order to clarify the reasons for the color formation, we measured and analyzed the changes in anthocyanins, phenolic compounds and expression of the key anthocyanin biosynthetic and regulatory genes in the colored process of ?ranny Smith?apples. The results indicated that two types of anthocyanins (i.e., cyanidin 3-O-galactoside and cyanidin 3-O-arabinoside) could be detected in the skin of ?ranny Smith?apples after bag removal. Cyanidin 3-O-galactoside accounted for most of the total anthocyanin contents and significantly accumulated within 7?days, along with the concentrations of chlorophylls and carotenoids significantly decreasing. The concentrations of catechin and procyanidin B2 in bagged apples also noticeably decreased, while the concentration of epicatechin increased. After bag removal, the expression of MdDFR, MdANS and MdUFGT increased sharply within 7?days. The transcription level of MdMYB10 and MdBHLH33 was markedly up-regulated and peaked at 1?day. While in the non-bagged apples, little anthocyanins were found and the expression of those genes showed low levels and remained stable. Our results suggested that ?ranny Smith?apples, in the special growing conditions of the Loess Plateau region of China, had the potential ability of coloration. And the higher expression of MdDFR, MdANS, MdUFGT, MdMYB10 and MdBHLH33 genes could enhance anthocyanin accumulation on ?ranny Smith?apples.