Extracellular DNA Inhibits Salmonella enterica Serovar Typhimurium and S. enterica Serovar Typhi Biofilm Development on Abiotic Surfaces

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• 作者：Hu Wang (1)
  Yang Huang (2)
  Shuyan Wu (1)
  Yuanyuan Li (1)
  Ying Ye (1)
  Yajie Zheng (1)
  Rui Huang (1)
  
• 刊名：Current Microbiology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：68
• 期：2
• 页码：262-268
• 全文大小：1,915 KB
• 作者单位：Hu Wang (1)
  Yang Huang (2)
  Shuyan Wu (1)
  Yuanyuan Li (1)
  Ying Ye (1)
  Yajie Zheng (1)
  Rui Huang (1)
  
  1. Medical College of Soochow University, No. 199, Ren Ai Road, Suzhou, 215123, Jiangsu, People鈥檚 Republic of China
  2. Lumigenex (Suzhou) Co. Ltd, 218 Xing Hu Road, Biobay Suite C24, Suzhou Industrial Park, Suzhou, 215123, People鈥檚 Republic of China
  
• ISSN：1432-0991

文摘

Extracellular DNA (eDNA) was identified and characterized in a 2-day-old biofilms developed by Salmonella enterica ser. Typhimurium SR-11 and S. enterica ser. Typhi ST6 using confocal laser scanning microscopy (CLSM) and enzymatic extraction methods. Results of microtitre plate assay and CLSM analysis showed both Salmonella strains formed significantly more biofilms in the presence of DNase I; Furthermore, a remarkable decrease of biofilm formation was observed when eDNA was added in the inoculation. However, for the pre-established biofilms on polystyrene and glass, no significant difference was observed between the DNase I treated biofilm and the corresponding non-treated controls. In conclusion, these results demonstrate that eDNA is a novel matrix component of Salmonella biofilms. This is the first evidence for the presence of eDNA and its inhibitive and destabilizing effect during biofilm development of S. enterica ser. Typhimurium and S. enterica ser. Typhi on abiotic surfaces.