MicroRNA-204-5p inhibits gastric cancer cell proliferation by downregulating USP47 and RAB22A

详细信息    查看全文

• 作者：Binbin Zhang (1)
  Yuan Yin (2)
  Yaling Hu (2)
  Jiwei Zhang (2)
  Zehua Bian (2)
  Mingxu Song (2)
  Dong Hua (2)
  Zhaohui Huang (1) (2)
  
• 关键词：Gastric cancer ; miR ; 204 ; 5p ; Cell proliferation ; USP47 ; RAB22A
• 刊名：Medical Oncology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：32
• 期：1
• 全文大小：2,662 KB
• 参考文献：1. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116(2):281-7. CrossRef
  2. He L, Hannon GJ. MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet. 2004;5(7):522-1. CrossRef
  3. Di Leva G, Garofalo M, Croce CM. MicroRNAs in cancer. Annu Rev Pathol. 2014;9:287-14. CrossRef
  4. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61(2):69-0. CrossRef
  5. Hou Z, Xie L, Yu L, Qian X, Liu B. MicroRNA-146a is down-regulated in gastric cancer and regulates cell proliferation and apoptosis. Med Oncol. 2012;29(2):886-2. CrossRef
  6. Li C, Li JF, Cai Q, Qiu QQ, Yan M, Liu BY, et al. MiRNA-199a-3p: a potential circulating diagnostic biomarker for early gastric cancer. J Surg Oncol. 2013;108(2):89-2. CrossRef
  7. Chen Y, Zuo J, Liu Y, Gao H, Liu W. Inhibitory effects of miRNA-200c on chemotherapy-resistance and cell proliferation of gastric cancer SGC7901/DDP cells. Chin J Cancer. 2010;29(12):1006-1. CrossRef
  8. Liu D, Xia P, Diao D, Cheng Y, Zhang H, Yuan D, et al. MiRNA-429 suppresses the growth of gastric cancer cells in vitro. J Biomed Res. 2012;26(5):389-3. CrossRef
  9. Sacconi A, Biagioni F, Canu V, Mori F, Di Benedetto A, Lorenzon L, et al. miR-204 targets Bcl-2 expression and enhances responsiveness of gastric cancer. Cell Death Dis. 2012;3:e423. CrossRef
  10. Li X, Zhang Y, Zhang H, Liu X, Gong T, Li M, et al. miRNA-223 promotes gastric cancer invasion and metastasis by targeting tumor suppressor EPB41L3. Mol Cancer Res. 2011;9(7):824-3. CrossRef
  11. Zhang L, Wang X, Chen P. MiR-204 down regulates SIRT1 and reverts SIRT1-induced epithelial-mesenchymal transition, anoikis resistance and invasion in gastric cancer cells. BMC Cancer. 2013;13:290. CrossRef
  12. Zhou X, Li L, Su J, Zhang G. Decreased miR-204 in H. pylori-associated gastric cancer promotes cancer cell proliferation and invasion by targeting SOX4. PLoS One. 2014;9(7):e101457. CrossRef
  13. Shin VY, Chu KM. MiRNA as potential biomarkers and therapeutic targets for gastric cancer. World J Gastroenterol. 2014;20(30):10432-. CrossRef
  14. Zhang R, Wang W, Li F, Zhang H, Liu J. MicroRNA-106b~25 expressions in tumor tissues and plasma of patients with gastric cancers. Med Oncol. 2014;31(10):243. CrossRef
  15. Huang Z, Huang D, Ni S, Peng Z, Sheng W, Du X. Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer. Int J Cancer. 2010;127(1):118-6. CrossRef
  16. Yin Y, Zhang B, Wang W, Fei B, Quan C, Zhang J, et al. miR-204-5p inhibits proliferation and invasion and enhances chemotherapeutic sensitivity of colorectal cancer cells by down-regulating RAB22A. Clin Cancer Res. 2014. doi:10.1158/1078-0432.CCR-14-1030 .
  17. Ying Z, Li Y, Wu J, Zhu X, Yang Y, Tian H, et al. Loss of miR-204 expression enhances glioma migration and stem cell-like phenotype. Cancer Res. 2013;73(2):990-. CrossRef
  18. Li W, Jin X, Zhang Q
• 作者单位：Binbin Zhang (1)
  Yuan Yin (2)
  Yaling Hu (2)
  Jiwei Zhang (2)
  Zehua Bian (2)
  Mingxu Song (2)
  Dong Hua (2)
  Zhaohui Huang (1) (2)
  
  1. Oncology Institute, The Fourth Affiliated Hospital of Soochow University, The Fourth People’s Hospital of Wuxi, Wuxi, 214062, Jiangsu Province, China
  2. Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, 200 Huihe Road, Wuxi, 214062, Jiangsu, China
  
• ISSN：1559-131X

文摘

MicroRNAs (miRNAs) are a type of small noncoding RNAs that are strongly implicated in carcinogenesis. However, the potential diagnostic, prognostic and therapeutic roles of the majority of miRNAs in the pathological processes of tumorigenesis remain largely unknown. Our and others-data revealed that miR-204-5p was significantly downregulated in gastrointestinal tumor tissues compared with adjacent noncancerous tissues. The downregulation of miR-204-5p was confirmed in our gastric cancer (GC) cohort, and we showed that ectopic expression of miR-204-5p inhibited, whereas silencing miR-204-5p expression promoted GC cell proliferation in vitro. Subsequent mechanistic investigations identified that USP47 and RAB22A are direct functional targets of miR-204-5p in GC. Silencing the expression of USP47 and RAB22A using siRNA phenocopied the proliferation-inhibiting function of miR-204-5p in GC cells. Our results uncovered that miR-204-5p acts as a tumor suppressor in GC through inhibiting USP47 and RAB22A.