A Comparative Analysis of the atp8 Gene Between a Cytoplasmic Male Sterile Line and Its Maintainer and Further Development of a Molecular Marker Specific to Male Sterile Cytoplasm in Kenaf (Hibiscus cannabinus L.)

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• 作者：Xiaofang Liao ; Yanhong Zhao ; Peng Chen ; Bujin Zhou…
• 关键词：Kenaf (Hibiscus cannabinus L.) ; CMS line and its maintainer line ; atp8 ; Molecular marker
• 刊名：Plant Molecular Biology Reporter
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：34
• 期：1
• 页码：29-36
• 全文大小：584 KB
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• 作者单位：Xiaofang Liao (1) (2)
  Yanhong Zhao (3)
  Peng Chen (1) (2)
  Bujin Zhou (1) (2)
  Yong Diao (1) (2)
  Mingli Yu (1) (2)
  Zhipeng Huang (1) (2)
  Ruiyang Zhou (1) (2)
  
  1. The Key Laboratory of Plant Genetic Breeding of Guangxi University, Nanning, 530005, Guangxi, People’s Republic of China
  2. College of Agriculture, Guangxi University, Nanning, 530005, Guangxi, People’s Republic of China
  3. Cash Crop Institute of Guangxi Academy of Agricultural Sciences, Nanning, 530007, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

In this study, the atp8 gene was cloned from the cytoplasmic male sterile (CMS) line UG93A and its maintainer line UG93B in kenaf. Its DNA sequence analysis showed that atp8 containing 480-bp, encoding 159 amino acid residues, and a 9-bp insertion was found at the 3′flanking sequence in UG93A compared with UG93B. The cDNA sequence of atp8 analyzed by RT-PCR indicated that there were five loci edited, but six loci edited in UG93B. The editing frequencies were higher in sterile cytoplasm than in fertile cytoplasm. The relative expression of atp8 analyzed by real-time PCR showed that the expressed level of atp8 in UG93A was lower than that of its maitainer UG93B and its F₁ hybrid UG93A/992 (a restore line). Furthermore, based on the difference of the 9-bp differences at the 3′flanking sequence of atp8 between UG93A and UG93B, a molecular marker specific to male sterile cytoplasm was developed, which can be used for indentifying whether any germplasm of kenaf is male sterile cytoplasm or male fertile cytoplasm. Keywords Kenaf (Hibiscus cannabinus L.) CMS line and its maintainer line atp8 Molecular marker