Antibacterial evaluation of flavonoid compounds against E. coli by microcalorimetry and chemometrics

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• 作者：Weijun Kong ; Yanling Zhao ; Xiaoyan Xing…
• 关键词：Flavonoid compounds ; E. coli ; Antibacterial evaluation ; Microcalorimetry ; Chemometrics
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：99
• 期：14
• 页码：6049-6058
• 全文大小：1,067 KB
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• 作者单位：Weijun Kong (1)
  Yanling Zhao (2)
  Xiaoyan Xing (1)
  Xinping Ma (3)
  Xiaojiao Sun (2)
  Meihua Yang (1)
  Xiaohe Xiao (2)
  
  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China
  2. China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing, 100039, China
  3. Department of Traditional Chinese Medicine, Air Force General Hospital, PLA, Beijing, 100142, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Fighting against multidrug-resistant bacteria requires reliable methods to evaluate the effect of antibacterial agents. As a universal, non-destructive, and highly sensitive tool, microcalorimetry has been used in many biological investigations to provide continuous real-time monitoring of the metabolic activity. This method, based on heat-flow output, was used to evaluate the influence of two flavonoid compounds (liquiritigenin and liquiritin) on Escherichia coli. Some crucial information, such as the thermogenic power-time curve and thermokinetic parameters of E. coli growth affected by the two compounds, was obtained and further studied by chemometric techniques including similarity analysis, multivariate analysis of variance, and principal component analysis. By comparing the values of two main parameters, k 2 (growth rate constant of the second exponential phase) and Q 1 (heat output of the first exponential growth phase) of E. coli based on the box and whisker plot, liquiritigenin and liquiritin could be differentiated according to their antibacterial effects; liquiritin with IC50 (half-inhibitory concentration) of 198.6?μg?mL? expressed a stronger antibacterial effect than liquiritigenin with IC50 of 337.8?μg?mL?. The glucoside group in liquiritin containing four additional free hydroxyls in the diphenylpropane skeleton was crucial for inducing the antibacterial effect. Liquiritin might be a promising candidate against E. coli. This study provides a valuable method for searching for novel antibacterial agents using microcalorimetry with chemometrics.