A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC–MS analysis

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• 作者：Yuwei Chang (1)
  Lei Zhang (2)
  Xin Lu (1)
  Chunxia Zhao (1)
  Zhen Zhu (2)
  Feng Wang (3)
  Junjie Zhang (1)
  Shili Chen (1)
  Yanni Zhao (1)
  Guowang Xu (1)
  
• 关键词：Methyl tert ; butyl ether ; Metabolomics ; Lipidomics ; Insecticide treatment ; Transgenic rice
• 刊名：Metabolomics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：10
• 期：6
• 页码：1197-1209
• 全文大小：1,757 KB
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• 作者单位：Yuwei Chang (1)
  Lei Zhang (2)
  Xin Lu (1)
  Chunxia Zhao (1)
  Zhen Zhu (2)
  Feng Wang (3)
  Junjie Zhang (1)
  Shili Chen (1)
  Yanni Zhao (1)
  Guowang Xu (1)
  
  1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China
  2. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, China
  3. Fujian Province Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China
  
• ISSN：1573-3890

文摘

Abiotic stress caused by insecticide treatment is an interesting and challenging topic in plant research. Here a simultaneous extraction method with methyl tert-butyl ether for metabolomics and lipidomics analysis by using rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. The extraction efficiency of lipidome and metabolome based on the current developed method showed an obvious improvement compared with literatures. About 300 metabolites were identified in rice leaf. Method validation was performed and analytical properties including the linearity (R2, 0.991-.000), repeatability (over 87?% peaks with CV?cry1Ac and sck genes and its wild counterpart. Antioxidants including ferulic acid and sinapic acid were down-regulated at 24?h after insecticide treatment. Signaling metabolites including salicylic acid and nicotinamide adenine dinucleotide were significantly up-regulated at 12-4?h after treatment in transgenic rice. More flavonoids were significantly changed in transgenic plants. Results indicated that gene transformation affected the metabolic response of rice to insecticide stress.