Acetylcholinesterase Biosensor Based on Poly (diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

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• 作者：Xia Sun ; Zhili Gong ; Yaoyao Cao ; Professor Xiangyou Wang
• 关键词：Biosensor ; Acetylcholinesterase ; Multi ; walled carbon nanotubes ; Graphene ; Poly (diallyldimethylammonium chloride)
• 刊名：Nano-Micro Letters
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：5
• 期：1
• 页码：47-56
• 全文大小：352KB
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• 作者单位：Xia Sun (18)
  Zhili Gong (18)
  Yaoyao Cao (18)
  Professor Xiangyou Wang (18)
  
  18. College of Agriculture and Food Engineering, Shandong University of Technology, Zibo, Shandong, 255049, China
  
• 刊物类别：Nanotechnology and Microengineering; Nanotechnology; Nanoscale Science and Technology;
• 刊物主题：Nanotechnology and Microengineering; Nanotechnology; Nanoscale Science and Technology;
• 出版者：Springer Berlin Heidelberg
• ISSN：2150-5551

文摘

In this paper, an amperometric acetylcholinesterase (AChE) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly (diallyldimethy-lammonium chloride) -multi-walled carbon nanotubes-graphene hybrid film was modified onto the glassy carbon electrode (GCE) surface, then AChE was immobilized onto the modified GCE to fabricate the AChE biosensor. The morphologies and electrochemistry properties of the prepared AChE biosensor were investigated by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Based on the inhibition of pesticides on the AChE activity, using carbaryl as model compounds, the biosensor exhibited low detection limit, good reproducibility and high stability in a wide range. Moreover, the biosensor can also be used for direct analysis of practical samples, which would provide a new promising tool for pesticide residues analysis. Keywords Biosensor Acetylcholinesterase Multi-walled carbon nanotubes Graphene Poly (diallyldimethylammonium chloride)