Assembly of a novel biosynthetic pathway for gentamicin B production in Micromonospora echinospora
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- 作者：Xianpu Ni ; Zhenpeng Sun ; Yawen Gu ; Hao Cui ; Huanzhang Xia
- 关键词：Micromonospora echinospora ; Gentamicin B ; Metabolic engineering ; Artificial biosynthetic pathway
- 刊名：Microbial Cell Factories
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：15
- 期：1
- 全文大小：1,709 KB
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Zhenpeng Sun (1)
Yawen Gu (1)
Hao Cui (1)
Huanzhang Xia (1)
1. School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, No.103 Wenhua Road, Shenyang, Liaoning, China - 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
Applied Microbiology
Environmental Engineering/Biotechnology - 出版者：BioMed Central
- ISSN：1475-2859
文摘
Background Isepamicin is a weakly toxic but highly active aminoglycoside antibiotic derivative of gentamicin B. Gentamicin B is a naturally occurring minor component isolated from Micromonospora echinospora. 2ʹ-NH2-containing gentamicin C complex is a dominant compound produced by wild-type M. echinospora; by contrast, 2ʹ-OH-containing gentamicin B is produced as a minor component. However, the biosynthetic pathway of gentamicin B remains unclear. Considering that gentamicin B shares a unique C2ʹ hydroxyl group with kanamycin A, we aimed to design a new biosynthetic pathway of gentamicin B by combining twelve steps of gentamicin biosynthesis and two steps of kanamycin biosynthesis.