Enhanced differentiation potential of human amniotic mesenchymal stromal cells by using three-dimensional culturing

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• 作者：Xue Lin (1)
  Hao Yu Li (2)
  Lian Feng Chen (1)
  Bo Jiang Liu (3)
  Yian Yao (4)
  Wen Ling Zhu (1)
  
• 关键词：Amniotic mesenchymal stromal cell ; Cell culture ; Fibrin sealant ; Cardiac lysates ; Mesenchymal stem cell ; Cardiomyocyte ; like cells ; Human
• 刊名：Cell and Tissue Research
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：352
• 期：3
• 页码：523-535
• 全文大小：914KB
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• 作者单位：Xue Lin (1)
  Hao Yu Li (2)
  Lian Feng Chen (1)
  Bo Jiang Liu (3)
  Yian Yao (4)
  Wen Ling Zhu (1)
  
  1. Department of Cardiology, Peking Union Medical College Hospital, ShuaiFuYuan 1#, East District, Beijing, 100730, China
  2. School of Life Science, SiChuan University, ChengDu, 610041, SiChuan, China
  3. Department of Cardiology, Third Hospital of TianJin, TianJin, 300203, China
  4. Department of Cardiology, ShangHai East Hospital, ShangHai, 200120, China
  
• ISSN：1432-0878

文摘

The therapeutic potential of human amniotic mesenchymal stromal cells (hAMSCs) remains limited because of their differentiation towards mesenchymal stem cells (MSCs) following adherence. The aim of this study was to develop a three-dimensional (3-D) culture system that would permit hAMSCs to differentiate into cardiomyocyte-like cells. hAMSCs were isolated from human amnions of full-term births collected after Cesarean section. Immunocytochemistry, immunofluorescence and flow cytometry analyses were undertaken to examine hAMSC marker expression for differentiation status after adherence. Membrane currents were determined by patch clamp analysis of hAMSCs grown with or without cardiac lysates. Freshly isolated hAMSCs were positive for human embryonic stem-cell-related markers but their marker profile significantly shifted towards that of MSCs following adherence. hAMSCs cultured in the 3-D culture system in the presence of cardiac lysate expressed cardiomyocyte-specific markers, in contrast to those maintained in standard adherent cultures or those in 3-D cultures without cardiac lysate. hAMSCs cultured in 3-D with cardiac lysate displayed a cardiomyocyte-like phenotype as observed by membrane currents, including a calcium-activated potassium current, a delayed rectifier potassium current and a Ca2+-resistant transient outward K+ current. Thus, although adherence limits the potential of hAMSCs to differentiate into cardiomyocyte-like cells, the 3-D culture of hAMSCs represents a more effective method of their culture for use in regenerative medicine.