Tumor cell detection device based on surface plasmon resonance imaging and image processing

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• 作者：Mengchao Yang (1)
  Xinyu Jin (2)
  Maokai Yuan (1)
  Fan Liu (2)
  Yicong Lang (2)
  Zhongyu Wu (1)
  Liancheng Xiang (1)
  Chao Zhou (1)
  Bo Ouyang (2)
  Wei Jin (1)
  Ying Mu (1)
  
• 关键词：Surface plasmon resonance ; Image processing ; Tumor detection ; Gravity induced
• 刊名：Chemical Research in Chinese Universities
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：30
• 期：2
• 页码：211-215
• 全文大小：466 KB
• 参考文献：1. He R., Lin C., Su Y., Chiu K., Chang N., Wu H., Chen S., / Opt. Express, 2010, / 18, 3649 CrossRef
  2. Chang C., Lin S., Lee C., Chuang T., Hsueh P., Lai H., Lin C., / Biosensors and Bioelectronics, 2012, / 37, 68 CrossRef
  3. Kai E., Sawata S., Ikebukuro K., Iida T., Honda T., Karube I., / Anal. Chem., 1999, / 71(4), 796 CrossRef
  4. Chen H., Gal Y., Kim S., Choi H., Oh M., Lee J., Koh K., / Sensors and Actuators B: Chemical, 2008, / 133, 577 CrossRef
  5. Tang L. J., Casas M., / Anal. Chem., 2013, / 85(3), 1431 CrossRef
  6. Horii M., Shinohara H., Iribe Y., Suzuki M., / Analyst, 2011, / 136, 2706 CrossRef
  7. Yanase Y., Hiragun T., Kaneko S., Gould H. J., Greaves M. W., / Biosensors and Bioelectronics, 2010, / 26, 674 CrossRef
  8. Hiragun T., Yanase Y., Kose K., Kawaguchi T., Uchida K., Tanaka S., Hide M., / Biosensors and Bioelectronics, 2012, / 32, 202 CrossRef
  9. Natarajan S., Katsamba P. S., Miles A., Eckman J., Papalia G. A., Rich R. L., Gale B., Myszka D. G., / Anal. Biochem., 2008, / 373, 141 CrossRef
  10. Eddings M. A., Eckman J. W., Arana C. A., Papalia G. A., Connolly J. E., Gale B. K., Myszka D. G., / Anal. Biochem., 2009, / 385, 309 CrossRef
  11. Nelson B. P., Grimsrud T. E., Liles M. R., Goodman R. M., Corn R. M., / Anal. Chem., 2000, / 73, 1 CrossRef
  12. Jiang J., Fan D. N., Chen W., Xiong Z. H., / Medical Information, 2009, / 22(1), 8
  13. You Y. R., Fan Y. L., Pang Q., / Computer Engineering and Applications, 2005, (20), 206
  14. Zhang Y., Jin W., Yang M. C., Zhang T. Q., Zhou C., Xie F., Song Q., Ren H., Jin Q. H., Mu Y., / Chem. Res. Chinese Universities, 2012, / 28(5), 792
  15. Zhan S., Wang X., Luo Z., Zhou H., Chen H., Liu Y., / Sensors and Actuators B: Chemical, 2011, / 153, 427 CrossRef
  16. Johnston K. S., Booksh K. S., Chinowsky T. M., Yee S. S., / Sensors and Actuators B: Chemical, 1999, / 54, 80 CrossRef
  17. Chinowsky T. M., Yee S. S., / Sensors and Actuators B: Chemical, 1998, / 51, 321 CrossRef
  18. Schuck P., Zhao H., / Methods in Molecular Biology, 2010, / 627, 15 CrossRef
  19. Bishop J., Blair S., / Biosensors and Bioelectronics, 2007, / 22, 2192 CrossRef
  20. Henry M. R., Stevens P. W., Sun J., Kelso D. M., / Anal. Biochem., 1999, / 2, 204 CrossRef
  21. Hagan M. F., Chakraborty A. K., / J. Chem. Phys., 2004, / 10, 4958 CrossRef
  22. Tawa K., Knoll W., / Nucleic Acids Res., 2004, / 8, 2372 CrossRef
  23. Fiche J. B., Buhot A., Calemczuk R., Livache T., / Biophys. J., 2007, / 3, 935 CrossRef
  24. Georgiadis R., Peterlinz K. P., Peterson A. W., / J. Am. Chem. Soc., 2000, / 122(13), 3166 CrossRef
  
• 作者单位：Mengchao Yang (1)
  Xinyu Jin (2)
  Maokai Yuan (1)
  Fan Liu (2)
  Yicong Lang (2)
  Zhongyu Wu (1)
  Liancheng Xiang (1)
  Chao Zhou (1)
  Bo Ouyang (2)
  Wei Jin (1)
  Ying Mu (1)
  
  1. Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, 310058, P. R. China
  2. Department of Information Science & Electronic Engineering, Zhejiang University, Hangzhou, 310027, P. R. China
  
• ISSN：2210-3171

文摘

A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging(SPRi) and image processing. In this device, a gravity-induced flow injection chip(gFIC) was exploited to replace a pump. Also two charge coupled devices(CCDs) were used to detect HepG2 cells by SPRi and image processing, respectively. The results of two CCDs are associated. Protein A was used to modify the sensing surface. The inlet angle was carefully adjusted for the device to get an enhanced image. In the test, the contrast among cell solutions at different concentrations can be easily distinguished. The other CCD using image processing can tell false-positive in some degree. This detection is label-free, real time, and precise.