Expression and clinical evidence of miR-494 and PTEN in non-small cell lung cancer

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• 作者：Juan Wang ; Hongtao Chen ; Yifeng Liao ; Nan Chen ; Tianze Liu ; Huitao Zhang…
• 关键词：NSCLC ; miR ; 494 ; PTEN ; In situ hybridization
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：36
• 期：9
• 页码：6965-6972
• 全文大小：801 KB
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• 作者单位：Juan Wang (1)
  Hongtao Chen (2)
  Yifeng Liao (1)
  Nan Chen (1)
  Tianze Liu (3)
  Huitao Zhang (4)
  Hongyu Zhang (1)
  
  1. Department of Medical Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, No.52, East Meihua Road, Zhuhai, Guangdong, People’s Republic of China, 519000
  2. Department of Laboratory, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China, 519000
  3. State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, China
  4. Department of Nephrology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China, 519000
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

The aim of this study was to explore the expression and clinical significance of miR-494 and PTEN (phosphatase and tensin homologue deleted on chromosome ten) in non-small cell lung cancer (NSCLC). Immunohistochemistry for PTEN and in situ hybridization (ISH) for miR-494 were performed in 92 NSCLC tissues and 10 normal lung tissues to detect their expression, and correlation between their expression with clinical characteristics and prognosis was analyzed. The expression of miR-494 was significantly higher in NSCLC than in normal lung tissues (P = 0.004). The positive expression of PTEN protein in the lung carcinoma tissues was significantly lower than that in the normal lung tissues (P = 0.013), while the level of miR-494 expression was negatively correlated with PTEN expression (r = −0.577, P < 0.01). The high positive rate of miR-494 was positively correlated with pathological TNM (p-TNM) staging and lymph node metastasis. The expression of miR-494 was negatively correlated with grade of differentiation. However, the expression of PTEN was positively correlated with grade of differentiation. Patients with over-expression of miR-494 had a shorter overall survival (OS), while the negative group of PTEN was correlated with poor OS. MiR-494 over-expression and low PTEN expression are closely related to tumor p-TNM staging and lymph node metastasis, differentiation, and OS. Combined detection of PTEN and miR-494 can aid in determining malignancy degree and the prognosis of patients with NSCLC. MiR-494 may be served as a novel prognostic factor and may lead to new treatment strategies for NSCLC.