Evidence for hydrogen-deuterium exchange in viral particles

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• 作者：Jiangsen Mao (1)
  Ziyang Liu (2)
  Caihua Tang (1)
  Yihui He (2)
  Jiahong Zhu (1)
  Chengyu Wang (3)
  Shaoai Chai (1)
  Yueqing Chen (1)
  Wen Qian (1)
  
• 关键词：gas ; isotope ; mass spectrometry ; virus thermostability ; heavy water (D2O) ; hydrogen ; deuterium exchange ; Japanese encephalitis virus ; hepatitis A virus ; viral RNA
• 刊名：Chinese Science Bulletin
• 出版年：2004
• 出版时间：February 2004
• 年：2004
• 卷：49
• 期：3
• 页码：253-257
• 全文大小：168KB
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• 作者单位：Jiangsen Mao (1)
  Ziyang Liu (2)
  Caihua Tang (1)
  Yihui He (2)
  Jiahong Zhu (1)
  Chengyu Wang (3)
  Shaoai Chai (1)
  Yueqing Chen (1)
  Wen Qian (1)
  
  1. Zhejiang Academy of Medical Sciences, 310013, Hangzhou, China
  2. Department of Chemistry, Zhejiang University, 310027, Hangzhou, China
  3. Chinese Academy of Geologic Sciences, 100037, Beijing, China
  
• ISSN：1861-9541

文摘

Heavy water (D2O) could enhance thermostability of some viruses. However, the underlying mechanism is not clear. Here we report the development of a matrix-aided gas-isotope-ratio mass spectrometry method that allows direct determination of deuterium/hydrogen (D/H) ratio in D2O-treated Japanese encephalitis virus (JEV), hepatitis A virus (HAV) and RNA from D2O-treated HAV. The D/H ratio was expressed as δDSMOW. Our experiments showed that δDSMOW values increased significantly in D2O-treated viral samples compared to normal controls, and increment in δDSMOW of D2O, treated viral samples was in a fine linear relationship with increment in amount of samples loaded in BSA matrix. Our experiments also indicated that increased δDSMOW of D2O-treated virus correlated well with its enhanced thermostability. The results suggested that hydrogen-deuterium exchange occurred in viral particles and its RNA structure as a result of D2O-treatment. Furthermore, such exchange could cause changes in viral phenotype, such as enhanced thermostability.