A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice

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• 作者：Hong Chen (1)
  Zhijun Cheng (2)
  Xiaoding Ma (2)
  Han Wu (1)
  Yanling Liu (1)
  Kunneng Zhou (1)
  Yilin Chen (1)
  Weiwei Ma (2)
  Jingcui Bi (2)
  Xin Zhang (2)
  Xiuping Guo (2)
  Jiulin Wang (2)
  Cailin Lei (2)
  Fuqing Wu (2)
  Qibing Lin (2)
  Yuqiang Liu (1)
  Linglong Liu (1)
  Ling Jiang (1)
  
• 关键词：Heme oxygenase ; 1 ; Map ; based cloning ; Yellow ; green leaves ; Chloroplast ; Chl biosynthesis ; Rice
• 刊名：Plant Cell Reports
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：32
• 期：12
• 页码：1855-1867
• 全文大小：
• 作者单位：Hong Chen (1)
  Zhijun Cheng (2)
  Xiaoding Ma (2)
  Han Wu (1)
  Yanling Liu (1)
  Kunneng Zhou (1)
  Yilin Chen (1)
  Weiwei Ma (2)
  Jingcui Bi (2)
  Xin Zhang (2)
  Xiuping Guo (2)
  Jiulin Wang (2)
  Cailin Lei (2)
  Fuqing Wu (2)
  Qibing Lin (2)
  Yuqiang Liu (1)
  Linglong Liu (1)
  Ling Jiang (1)
  
  1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China
  2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing, 100081, China
  
• ISSN：1432-203X

文摘

Key message An insert mutation of YELLOW - GREEN LEAF2 , encoding Heme Oxygenase 1 , results in significant reduction of its transcript levels, and therefore impairs chlorophyll biosynthesis in rice. Abstract Heme oxygenase (HO) in higher plants catalyzes the degradation of heme to synthesize phytochrome precursor and its roles conferring the photoperiodic control of flowering in rice have been revealed. However, its involvement in regulating rice chlorophyll (Chl) synthesis is not fully explored. In this study, we isolated a rice mutant named yellow-green leaf 2 (ygl2) from a 60Co-irradiated population. Normal grown ygl2 seedlings showed yellow-green leaves with reduced contents of Chl and tetrapyrrole intermediates whereas an increase of Chl a/b ratio. Ultrastructural analyses demonstrated grana were poorly stacked in ygl2 mutant, resulting in underdevelopment of chloroplasts. The ygl2 locus was mapped to chromosome 6 and isolated via map-based cloning. Sequence analysis indicated that it encodes the rice HO1 and its identity was verified by transgenic complementation test and RNA interference. A 7-Kb insertion was found in the first exon of YGL2/HO1, resulting in significant reduction of YGL2 expressions in the ygl2 mutant. YGL2 was constitutively expressed in a variety of rice tissues with the highest levels in leaves and regulated by temperature. In addition, we found expression levels of some genes associated with Chl biosynthesis and photosynthesis were concurrently altered in ygl2 mutant. These results provide direct evidence that YGL2 has a vital function in rice Chl biosynthesis.