cDNA-AFLP analysis on 2 Osmanthus fragrans cultivars with different flower color and molecular characteristics of OfMYB1 gene

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• 作者：Yuanji Han ; Weicai Chen ; Fengbo Yang ; Xiaohui Wang ; Meifang Dong ; Ping Zhou…
• 关键词：Osmanthus fragrans ; cDNA ; AFLP ; Transcript ; derived fragments ; MYB transcription factor
• 刊名：Trees - Structure and Function
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：29
• 期：3
• 页码：931-940
• 全文大小：2,922 KB
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• 作者单位：Yuanji Han (1)
  Weicai Chen (1)
  Fengbo Yang (1)
  Xiaohui Wang (1)
  Meifang Dong (1)
  Ping Zhou (1)
  Fude Shang (1)
  
  1. Key Laboratory of Plant Stress Biology, State Key Laboratory of Cotton Biology, Laboratory of Plant Germplasm and Genetic Engineering, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Forestry
  Plant Sciences
  Agriculture
  Plant Anatomy and Development
  Plant Pathology
  Plant Physiology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-2285

文摘

Key message In this study, we carried out cDNA-AFLP analysis on petals of 2 Osmanthus fragrans cultivars, yielding 102 cDNA fragments. OfMYB1 gene was cloned and the molecular characteristics were studied. Abstract The Osmanthus fragrans cultivars ‘Dangui-and ‘Yingui-differ considerably in floral coloration and volatiles. We carried out cDNA-AFLP analysis on petals of the two cultivars to isolate genes involved in the production of floral pigments and aromatic chemicals. Using 256 primer combinations, we detected 390 differentially expressed fragments, yielding 102 cDNA fragments. Of the differentially expressed transcripts, 45 were upregulated in ‘Yingui-and 57 were upregulated in ‘Dangui- The differentially expressed genes were involved in several metabolic and signal transduction pathways. According to the cDNA-AFLP analysis, a 72-bp fragment was upregulated in ‘Dangui- sequence comparison of the differentially expressed transcript-derived fragment showed this to be a MYB transcription factor. The cDNA of OfMYB1 was 749?bp long and contained a coding sequence of 585?bp encoding a polypeptide of 195 amino acids. A phylogenetic analysis placed the OfMYB1 gene within the subgroup containing “nyWsV/_M E/_DDlWP/_S-motif. Protoplast transformation experiment showed that OfMYB1 protein accumulated in cell nuclei. Yeast one hybrid assay confirmed that OfMYB1 acted as a transcriptional activator to regulate expression of OfPAL gene. The differential expression patterns of OfMYB1 and anthocyanin biosynthetic pathway genes (OfCHS, OfCHI, OfDFR and OfANS) in the 2 O. fragrans cultivars suggested that OfMYB1 may not regulate the expression of these genes.