Molecular cloning and expression of 17β-hydroxysteroid dehydrogenase type 2 gene in Hu sheep
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- 作者：Chang Long Wang (1)
Shi Jia Ying (1) (2)
Zi Yu Wang (1)
Hui Jun Xing (1)
Li Zhong Wang (1)
Dong Yang He (1)
Shen Hua Xiao (3)
Feng Wang (1) - 关键词：17β ; HSD2 ; Tissue expression analysis ; Hu sheep
- 刊名：Molecular Biology Reports
- 出版年：2013
- 出版时间：February 2013
- 年：2013
- 卷：40
- 期：2
- 页码：1073-1080
- 全文大小：546KB
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Shi Jia Ying (1) (2)
Zi Yu Wang (1)
Hui Jun Xing (1)
Li Zhong Wang (1)
Dong Yang He (1)
Shen Hua Xiao (3)
Feng Wang (1)
1. Institute of Sheep & Goat Science, and Center of Animal Embryo Engineering & Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China
2. Institute of Animal Science, Jiangsu Academy of Agricultural Science, Nanjing, 210014, Jiangsu, People’s Republic of China
3. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China - ISSN：1573-4978
文摘
17β-Hydroxysteroid dehydrogenase type 2 (17β-HSD2) catalyzes the NADP+-dependent oxidation of the most potent estrogen 17β-estradiol into the weak estrogen estrone, and the conversion of testosterone to androstenedione. It has been reported that 17β-HSD2 was expressed in many tissues in human, rats, however, the full-length sequence of 17β-HSD2 gene and its expression in ewe were still unknown. In this study, we cloned the full-length cDNA sequence and investigated mRNA differential expression in 28 tissues of 12 adult Hu-Sheep which were fed with high- and low- dietary intake. The 1,317?bp full-length cDNA sequence was first cloned. The coding region was 1,167?bp in length, and the monomer was estimated to contain 389 amino acid residues. It shares high AA sequence identity with that of bos Taurus (96.13?%), sus scrofa (77.06?%), canis lupus familiaris (70.44?%), Callithrix jacchus (65.72?%), Nomascus leucogenys (65.46?%), pan troglodytes (65.21?%), human (64.69?%), mus musculus (58.35?%), and a comparatively lower identity to danio rerio (37.85?%). 17β-HSD2 gene was high expressed in gastrointestinal (GI) tract, liver, but weakly expressed in other tissues. No detected expression was examined in lung. 17β-HSD2 gene expression was significantly difference in rumen, omasum, duodenum, cecum, hypophysis after high- and low- dietary intake. Results from the present study suggested that 17β-HSD2 plays a crucial role in almost all tissues protecting against excessive levels of active steroid hormone, and GI tract maybe an important steroid hormone metabolizing organ in Hu-Sheep. This present study is the first to provide the primary foundation for further insight into this ovine gene.