Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi

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• 作者：Yingxue Qin (1) (2)
  Qingpi Yan (1) (2)
  Yongquan Su (3)
  Haiping Li (1) (2)
  Wenzheng Zou (1) (2)
  
• 关键词：chemotactic motility ; Vibrio harveyi ; transposon ; virulence
• 刊名：Acta Oceanologica Sinica
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：32
• 期：8
• 页码：55-60
• 全文大小：229KB
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• 作者单位：Yingxue Qin (1) (2)
  Qingpi Yan (1) (2)
  Yongquan Su (3)
  Haiping Li (1) (2)
  Wenzheng Zou (1) (2)
  
  1. Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, 361021, China
  2. Fisheries College, Jimei University, Xiamen, 361021, China
  3. College of Ocean and Earth Science, Xiamen University, Xiamen, 361005, China
  
• ISSN：1869-1099

文摘

Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (Gen Bank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (Gen Bank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth, motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energy metabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotactic motility of V. harveyi.