Isolation, complete genome sequencing, and phylogenetic analysis of the first Chuzan virus in China

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• 作者：Fang Wang ; Jun Lin ; Jitao Chang ; Yingying Cao ; Shaomin Qin ; Jianmin Wu…
• 关键词：Chuzan virus ; Isolation in China ; Complete genome ; Phylogenetic analysis
• 刊名：Virus Genes
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：52
• 期：1
• 页码：138-141
• 全文大小：362 KB
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• 作者单位：Fang Wang (1)
  Jun Lin (2)
  Jitao Chang (1)
  Yingying Cao (2)
  Shaomin Qin (2)
  Jianmin Wu (2)
  Li Yu (1)
  
  1. Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of China
  2. Guangxi Veterinary Research Institute, Nanning, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X

文摘

A Chuzan virus (CHUV), defined as GX871 here, was isolated from blood from a sentinel cattle firstly in China, and its full-length genome was sequenced in this study. The GX871 genome included 10 segments and 18914 bp, one base fewer than the CHUV prototype strain K-47 due to a one-base deletion in the 5′ non-coding region of segment 8. A frameshift mutation was detected in a short coding region (1010–1026 nt) corresponding to the VP1 protein; this frameshift resulted in a five-amino acid mutation from ₃₃₆CVLSY₃₄₀ to ₃₃₆YGAKL₃₄₀. In addition, there were a one-base deletion at 1713 nt and a one-base insertion at 1682 nt in the 3′ non-coding region of segment 5. Based on phylogenetic analysis of the deduced VP2 amino acid sequences, Palyam serogroup viruses were classified into three groups. The Chinese CHUV isolate GX871 was categorized into the same group as CHUV prototype strain K-47. The phylogenetic tree was divided into three clusters according to the geographical distribution of the partial nucleotide sequences of VP7, and this arrangement might define the geographical gene pool of CHUV. Keywords Chuzan virus Isolation in China Complete genome Phylogenetic analysis