Transcription Analysis of Genes Encoding the Wheat Root Transporter NRT1 and NRT2 Families During Nitrogen Starvation
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- 作者：Tiancai Guo (1)
Hongmei Xuan (1)
Yingying Yang (2)
Lina Wang (1)
Liting Wei (2)
Yonghua Wang (1)
Guozhang Kang (3) - 关键词：Nitrogen starvation ; Nitrate transporter ; Transcription level ; Triticum aestivum
- 刊名：Journal of Plant Growth Regulation
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：33
- 期：4
- 页码：837-848
- 全文大小：1,415 KB
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Lina Wang (1)
Liting Wei (2)
Yonghua Wang (1)
Guozhang Kang (3)
1. The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou, 450002, China
2. The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, 450002, China
3. The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, #62, Nongye Road, Zhengzhou, 450002, Henan Province, China - ISSN：1435-8107
文摘
In the present study, nitrogen (N) starvation for 8?days significantly inhibited the growth of wheat seedlings as manifested by decreased plant height, shoot fresh weight, and shoot dry weight, although it stimulated root growth. The nitrate and protein contents were markedly reduced and the oxidative stress marker, malondialdehyde content, was markedly increased in the leaves and roots of wheat seedlings during N starvation. The genes encoding the NRT1 and NRT2 families in bread wheat (Triticum aestivum L.) were identified, and their transcription levels were measured using quantitative real-time polymerase chain reaction in the roots of N-starved wheat seedlings. N starvation significantly enhanced the transcription levels of TaNRT1.1 at 2 and 4?days; TaNRT1.3 at 2, 4, and 6?days; TaNRT1.4 at 2?days; TaNRT1.7 and TaNRT1.8 at 2?days; TaNRT2.1 and TaNRT2.2 at 2?days; and TaNRT2.3 at 2 and 4?days. However, the TaNRT1.5 and TaNRT2.4 genes were greatly inhibited at all sampling time points after N starvation, whereas the TaNRT1.2 and TaNRT2.5 genes were dramatically induced. The functions of these transporters in N starvation of wheat seedlings based on these expression profiles are herein discussed.