Use of a whole-cell bioreporter, Acinetobacter baylyi, to estimate the genotoxicity and bioavailability of chromium(VI)-contaminated soils

详细信息    查看全文

• 作者：Bo Jiang ; Di Zhu ; Yizhi Song ; Dayi Zhang ; Zengjun Liu ; Xu Zhang…
• 关键词：Acinetobacter baylyi ; Bioavailability ; Chromium(VI) ; Contaminated soils ; Genotoxicity ; Whole ; cell bioreporters
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：37
• 期：2
• 页码：343-348
• 全文大小：351 KB
• 参考文献：1. Abollino O, Aceto M, Malandrino M, Sarzanini C, Mentasti E (2003) Adsorption of heavy metals on Na-montmorillonite. Effect of pH and organic substances. Water Res 37:1619-627 CrossRef
  2. Ackerley DF, Barak Y, Lynch SV, Curtin J, Matin A (2006) Effect of chromate stress on / Escherichia coli K-12. J Bacteriol 188:3371-381 CrossRef
  3. Anderson RA, Bryden NA, Polansky MM (1997) Lack of toxicity of chromium chloride and chromium picolinate in rats. J Am Coll Nutr 16:273-79 CrossRef
  4. Bao SQ (2008) Soil agrochemical analysis, 3rd edn. Agricultural Publishing House of China, Beijing
  5. Huang WE, Wang H, Zheng HJ, Huang LF, Singer AC, Thompson I, Whiteley AS (2005) Chromosomally located gene fusions constructed in / Acinetobacter sp. ADP1 for the detection of salicylate. Environ Microbiol 7:1339-348 CrossRef
  6. Ivask A, Fran?ois M, Kahru A, Dubourguier HC, Virta M, Douay F (2004) Recombinant luminescent bacterial sensors for the measurement of bioavailability of cadmium and lead in soils polluted by metal smelters. Chemosphere 55:147-56 CrossRef
  7. Liao VHC, Chien MT, Tseng YY, Ou KL (2006) Assessment of heavy metal bioavailability in contaminated sediments and soils using green fluorescent protein-based bacterial biosensors. Environ Pollut 142:17-3 CrossRef
  8. Llagostera M, Garrido S, Guerrero R, Barbé J (1986) Induction of SOS genes of / Escherichia coli by chromium compounds. Environ Mutagen 8:571-77 CrossRef
  9. Marqués MJ, Salvador A, Morales-Rubio A, de la Guardia M (2000) Chromium speciation in liquid matrices: a survey of the literature. Fresenius J Anal Chem 367:601-13 CrossRef
  10. Petrilli FL, Flora SD (1977) Toxicity and mutagenicity of hexavalent chromium on / Salmonella typhimurium. Appl Environ Microbiol 33:805-09
  11. Song Y et al (2014) A whole-cell bioreporter approach for the genotoxicity assessment of bioavailability of toxic compounds in contaminated soil in China. Environ Pollut. doi:10.1016/j.envpol.2014.08.024
  12. Song YZ, Li GH, Thornton SF, Thompson LP, Banwart SA, Lerner DN, Huang WE (2009) Optimization of bacterial whole cell bioreporters for toxicity assay of environmental samples. Environ Sci Technol 43:7931-938 CrossRef
  13. van der Meer JR, Belkin S (2010) Where microbiology meets microengineering: design and applications of reporter bacteria. Nat Rev Microbiol 8:511-22 CrossRef
  14. Wazne M, Moon DH, Jagupilla SC, Jagupilla SC, Christodoulatos C, Dermatas D, Chrysochoou M (2007) Remediation of chromite ore processing residue using ferrous sulfate and calcium polysulfide. Geosci J 11:105-10 CrossRef
  15. Zhang DY, Fakhrullin RF, ?zmen M, Wang H, Wang J, Paunov VN, Li GH, Huang WE (2011) Functionalization of whole-cell bacterial reporters with magnetic nanoparticles. Microb Biotechnol 4:89-7 CrossRef
  16. Zhang DY, He Y, Wang Y, Wang H, Wu L, Aries E, Huang WE (2012) Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. Microb Biotechnol 5:87-7 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

A whole-cell bioreporter, Acinetobacter baylyi ADPWH_recA, was used to estimate the genotoxicity and bioavailability of chromium (VI) [Cr(VI)] in contaminated soils. Upon direct exposure to pre-sonicated soil samples, ADPWH_recA gave the highest response to the genotoxicity of Cr(VI) within 5?h with a detection limit of 2?μM Cr(VI). Investigations on sites contaminated with Cr(VI) revealed that soil-associated Cr(VI) was bioavailable to the bioreporter although it could not be extracted into the aqueous phase. The physical and chemical properties of soil might influence the bioavailability of Cr(VI), and higher genotoxicity was found in soils with a lower pH. This whole cell bioreporter approach makes it feasible to evaluate the bioavailability and genotoxicity of Cr(VI)-contaminated soils to uncover their potential impact on human health.