Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70

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• 作者：Wenting Zhang ; Chengzhi Cai ; Li Lin ; Yizhi Jane Tao ; Meilin Jin
• 关键词：Infectious salmon anemia virus ; Subcellular localization ; Protein interaction ; Matrix protein ; Nuclear export protein ; Heat shock cognate 70
• 刊名：Virology Journal
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：14
• 期：1
• 全文大小：1528KB
• 刊物主题：Virology;
• 出版者：BioMed Central
• ISSN：1743-422X
• 卷排序：14

文摘

BackgroundInfectious salmon anemia virus (ISAV) is an important fish pathogen that causes high mortality in farmed Atlantic salmon. The ISAV genome consists of eight single-stranded, negative-sense RNA segments. The six largest segments contain one open reading frame (ORF) each, and encode three polymerase proteins, nucleoprotein, fusion protein, and hemagglutinin esterase protein. The two smallest segments contain more than one ORF each. The segment 7 encodes non-structural protein 1 (NS1) and nuclear export protein (NEP), while segment 8 encodes matrix protein 1 and 2 (M1 and M2). NS1 and M2 have been well known as antagonist of type I interferon. However, little is known about the characterization of M1 or NEP. In addition, heat shock cognate 70 (Hsc70) has been reported to interact with M1 and NEP of influenza viruses for the export of viral ribonucleoprotein (vRNP) via vRNP-M1-NEP complex, the goal of this study therefore was to characterize the subcellular localization and interactions of ISAV M1 and NEP as well as cellular Hsc70.