Discordance between in silico & in vitro analyses of ACE inhibitory & antioxidative peptides from mixed milk tryptic whey protein hydrolysate

详细信息    查看全文

• 作者：Alok Chatterjee ; S. K. Kanawjia ; Yogesh Khetra…
• 关键词：In silico ; In vitro ; Antihypertensive ; Antioxidant ; Whey protein
• 刊名：Journal of Food Science and Technology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：52
• 期：9
• 页码：5621-5630
• 全文大小：362 KB
• 参考文献：Adler-Nissen J (1986) In enzymatic hydrolysis of food proteins. Elsevier Applied Science Publishers, London, pp 32-5
  Ames BN, Shigenaga MK, Hagen TM (1993) In: Oxidants, antioxidants, and the degenerative diseases of aging, vol 90. Proceedings of National Academy of Sciences, USA, pp 7915-922
  Chang YW, Alli I (2012) In silico assessment: suggested homology of chickpea (Cicer arietinum L.) legumin and prediction of ACE inhibitory peptides from chickpea proteins using BLAST and BIOPEP analyses. Food Res Int. doi:10.-016/?j.?foodres.-012.-7.-06
  Cheung H-S, Wang F-L, Ondetti MA, Sabo EF, Cushman DW (1980) Binding of peptide substrates and inhibitors of angiotensinconverting enzyme. J Biol Chem 255:401-07
  Chobert JM, El-zahar K, Sitohy M, Dalgalarrondo M, Metro F, Choiset Y, Haertle T (2005) Angiotensin I converting enzyme (ACE) inhibitory activity of tryptic peptides of ovine beta lactoglobulin and of milk yoghurts obtained by using different starters. Lait 85:141-52CrossRef
  Cushman DW, Cheung HS (1971) Spectrophotometric assay and properties of angiotensin converting enzyme of Rabbit lung. Biochem Pharmacol 20(7):1637-648CrossRef
  Dalsgaard TK, Heegaard CW, Larsen LB (2008) Plasmin digestion of photooxidized milk proteins. J Dairy Sci 91:2175-183CrossRef
  Dziuba J, Niklewicz M, Iwaniak A, Darewicz M, Minkiewicz P (2004) Bioinformatic aided prediction for release possibilities of bioactive peptides from plant proteins. Acta Aliment 33(3):227-35CrossRef
  Dziuba M, Dziuba B, Iwaniak A (2009) Milk proteins as precursors of bioactive peptides. ACTA Sci Pol Technol Aliment 8(1):71-0
  Hernandez-ledesma B, Miralles B, Amigo L, Ramos M, Recio I (2005) Identification of antioxidant and ACE inhibitory peptides in fermented milk. J Sci Food Agric 85(6):1041-048CrossRef
  Kamau SM, Lu RR (2010) The effect of enzymes and hydrolysis conditions on degree of hydrolysis and DPPH radical scavenging activity of whey protein hysrolysates. Curr Res Dairy Sci 1994-434:1-
  Konard B, Dabrowska A, Szo?tysik M, Pokora M, Zambrowicz A, Chrzanowska J (2014) The evaluation of dipeptidyl peptidase (DPP)-IV, a-glucosidase and angiotensin converting enzyme (ACE) inhibitory activities of whey proteins hydrolyzed with serine protease isolated from Asian pumpkin (Cucurbita ficifolia). Int J Pept Res Ther. doi:10.-007/?s10989-014-9413-0
  Li G-H, Le G-W, Shi Y-H, Shrestha S (2004) Angiotensin I-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects. Nutr Res 24:469-86CrossRef
  Lopez-Fandino R, Otte J, Van-Camp J (2006) Physiological, chemical and technological aspects of milk protein derived peptides with antihypertensive and ACE-inhibitory activity. Int Dairy J. doi:10.-016/?j.?idairyj.-006.-6.-04
  Madureira AR, Tavares T, Gomes AMP, Pintado ME, Malcata FX (2010) Invited review: physiological properties of bioactive peptides obtained from whey proteins. J Dairy Sci 93:437-55CrossRef
  Mullally MM, Meisel H, FitzGerald RJ (1997) Identification of a novel angiotensin I converting enzyme inhibitory peptide corresponding to a tryptic fragment of bovine ? lactoglobulin. FEBS Lett 402:99-01CrossRef
  Otte J, Shalabya SM, Zakora M, Pripp AH, El-Shabrawyb SA (2007) Angiotensin-converting enzyme inhibitory activity of milk protein hydrolysates: Effect of substrate, enzyme and time of hydrolysis. Int Dairy J 17:488-03CrossRef
  Pellegrini A, Dettling C, Thomas U, Hunziker P (2001) Isolation and characterization of four bactericidal domains in the bovine ? lactoglobulin. Biochim Biophys Acta 1526:131-40CrossRef
  Pellegrini A, Thomas U, Bramaz N, Hunziker P, Fellenberg RV (1999) Isolation and identification of three bactericidal domains in the bovine α lactalbumin molecule. Acta Biochim Biophys Sin 1426(3):439-48CrossRef
  Pihlanto-leppala A, Koskinen P, Piilola K, Tupasela T, Korhonen H (2000) Angiotensin I converting enzyme inhibitory properties of whey protein digests: Concentration and characterization of active peptides. J Dairy Res 67:53-4CrossRef
  Pripp AH (2005) Initial proteolysis of milk proteins and its effect on formation of ACE inhibitory peptides during gastrointestinal proteolysis: a bioinformatic, in silico, approach. Eur Food Res Technol 221(5):712-16CrossRef
  Pripp AH, Sorensen R, Stepaniak L, Sorhaug T (2006) Relationship between proteolysis and angiotensin-I-converting enzyme inhibition in different cheeses. LWT Food Sci Technol 36:677-83CrossRef
  Rodriguez-carrio J, Fernandez A, Riera FA, Suarez A (2014) Immunomodulatory activities of whey ? lactoglobulin tryptic digested fractions. Int Dairy J 34(1):65-3CrossRef
  Roufik S, Gauthier SF, Dufour E, Turgeon SL (2006) Interactions between bovine β Lactoglobulin A and Various bioactive peptides as studied by front face fluorescence spectroscopy. J Agric Food Chem 54(14):4962-969CrossRef
  Shimada K, Fujikawa K, Yahara K, Nakamura T (1
• 作者单位：Alok Chatterjee (1)
  S. K. Kanawjia (1)
  Yogesh Khetra (1)
  Prerna Saini (2)
  
  1. Dairy Technology Division, National Dairy Research Institute, Karnal, 132001, Haryana, India
  2. Dairy Chemistry Division, National Dairy Research Institute, Karnal, 132001, Haryana, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nutrition
  Food Science
  Chemistry
  
• 出版者：Springer India
• ISSN：0975-8402

文摘

ACE inhibitory and antioxidative peptides identified by LCMS/MS, from mixed milk (Bubalus bubalis and Bos taurus) tryptic whey protein hydrolysate, were compared with the in silico predictions. α la and ? lg sequences, both from Bubalus bubalis and Bos taurus, were used for in silico study. SWISS-PROT and BIOPEP protein libraries were accessed for prediction of peptide generation. Study observed gaps in the prediction versus actual results, which remain unaddressed in the literature. Many peptides obtained in vitro, were not reflected in in silico predictions. Differences in identified peptides in separate libraries were observed too. In in silico prediction, peptides with known biological activities were also not reflected. Predictions, towards generation of bioactive peptides, based upon in silico release of proteins and amino acid sequences from different sources and thereupon validation in relation to actual results has often been reported in research literature. Given that computer aided simulation for prediction purposes is an effective research direction, regular updating of protein libraries and an effectual integration, for more precise results, is critical. The gaps addressed between these two techniques of research, have not found any address in literature. Inclusion of more flexibility with the variables, within the tools being used for prediction, and a hierarchy based database with search options for various peptides, will further enhance the scope and strength of research. Keywords In silico In vitro Antihypertensive Antioxidant Whey protein