Autoantibodies Against an Extracellular Peptide of the GluR3 Subtype of AMPA Receptors Activate Both Homomeric and Heteromeric AMPA Receptor Channels

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• 作者：Katayun Cohen-Kashi Malina ; Yonatan Ganor ; Mia Levite and Vivian I. Teichberg
• 关键词：Glutamate receptors ; GluR3 ; Autoantibodies ; Epilepsy ; Whole cell recording ; Oocytes ; AMPA receptor ; Brain
• 刊名：Neurochemical Research
• 出版年：2006
• 出版时间：October, 2006
• 年：2006
• 卷：31
• 期：10
• 页码：1181-1190
• 全文大小：264 KB

文摘

Autoantibodies to the GluR3-subtype of AMPA/glutamate receptors are found in the sera and cerebrospinal fluid of some individuals with epilepsy. They could possibly play a role in the pathophysiology of epilepsy since anti-GluR3 sera display glutamatergic agonist activity. We have investigated here the ability of affinity-purified antibodies (Abs) directed against the immunogenic peptide GluR3B (amino-acid 372–395) to interact with and activate recombinant GluR3-receptor channels expressed by Xenopus oocytes. We report here that the affinity-purified anti-GluR3B Abs directly activate GluR3-containing homomeric and heteromeric AMPA receptor complexes without the requirement of neuronal, glial or blood ancillary molecules. We present some of the properties of the purified anti-GluR3B Abs and discuss the possible physiological or pathological consequences of their activation of glutamate receptors.