Overexpression of geraniol 10-hydroxylase from Panax ginseng conferred enhanced resistance to Pseudomonas syringae in Arabidopsis

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• 作者：Sri Renuka Devi Balusamy ; Shadi Rahimi ; Yong-Gu Cho&#8230
• 关键词：Cytochrome P450 ; Geraniol 10 ; hydroxylase ; Panax ginseng ; Terpenoid indole alkaloid
• 刊名：Plant Growth Regulation
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：81
• 期：2
• 页码：305-316
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Anatomy/Development; Plant Physiology; Agriculture;
• 出版者：Springer Netherlands
• ISSN：1573-5087
• 卷排序：81

文摘

Geraniol, the precursor of terpenoid indole alkaloids can be converted to the 10-hydroxy geraniol by the function of geraniol 10-hydroxylase. In our study, for the first time, a full-length cytochrome P450 monooxygenase (P450) geraniol 10-hydroxylase (PgCYP76C9) cDNA was isolated and characterized from Panax ginseng Meyer. The gene has an open reading frame (ORF) of 1503 base pairs and encodes a precursor protein of 501 amino acids residues. The calculated molecular mass of the protein is approximately 56.3 kDa with a predicated isoelectric point of 8.45. Amino acid identities between PgG10H and other P450s of the CYP76 family in the database had revealed that the deduced amino acid of PgG10H sharing a higher sequence homology with geraniol 10-hydroxylase-like proteins encoded by Cinchona calisaya and Lonicera japonica. We implemented a molecular modeling method to evaluate the possible interaction of geraniol with PgG10H active site. Our finding showed that the geraniol was the potential ligand for PgG10H in P. ginseng. Expression of PgG10H gene was tissue-regulated and showed high expression in 3-year-old ginseng flowers and roots. Expression of PgG10H was differentially induced in ginseng, not only during Pseudomonas syringae infection and wounding but also after exposure to methyl jasmonate and salt stress. Furthermore, overexpression of the newly identified ginseng geraniol 10-hydroxylase P450 gene in Arabidopsis caused terpenoid indole alkaloid dihydrositsirikine production and also conferred enhanced resistance to P. syringae.