In vitro effects of N-acetyl cysteine alone and in combination with antibiotics on Prevotella intermedia

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• 作者：Ji-Hoi Moon ; Eun-Young Jang ; Kyu Sang Shim ; Jin-Yong Lee
• 关键词：Prevotella intermedia ; N ; acetyl cysteine ; biofilm ; modulator of antibiotic activity ; oral pathogen
• 刊名：Journal of Microbiology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：53
• 期：5
• 页码：321-329
• 全文大小：905 KB
• 参考文献：Albesa, I., Becerra, M.C., Battan, P.C., and Paez, P.L. 2004. Oxidative stress involved in the antibacterial action of different antibiotics. Biochem. Biophys. Res. Commun. 317, 605-09.View Article PubMed
  Ali, K.F. 2014. Estimation and evaluation of the effect of pH on ciprofloxacin in drug formulations. J. Chem. Pharm. Res. 6, 910-16.
  Alkawareek, M.Y., Algwari, Q.T., Laverty, G., Gorman, S.P., Graham, W.G., O’Connell, D., and Gilmore, B.F. 2012. Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure nonthermal plasma. PLoS One 7, e44289.View Article
  Andrés, M.T., Chung, W.O., Roberts, M.C., and Fierro, J.F. 1998. Antimicrobial susceptibilities of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens spp. isolated in Spain. Antimicrob. Agents Chemother. 42, 3022-023.PubMed Central PubMed
  Ashimoto, A., Chen, C., Bakker, I., and Slots, J. 1996. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol. Immunol. 11, 266-73.View Article PubMed
  Barbee, L.A., Soge, O.O., Holmes, K.K., and Golden, M.R. 2014. In vitro synergy testing of novel antimicrobial combination therapies against Neisseria gonorrhoeae. J. Antimicrob. Chemother. 69, 1572-578.View Article PubMed
  Becerra, M.C. and Albesa, I. 2002. Oxidative stress induced by ciprofloxacin in Staphylococcus aureus. Biochem. Biophys. Res. Commun. 297, 1003-007.View Article PubMed
  Bolivar, I., Whiteson, K., Stadelmann, B., Baratti-Mayer, D., Gizard, Y., Mombelli, A., Pittet, D., and Schrenzel, J. 2012. Bacterial diversity in oral samples of children in Niger with acute noma, acute necrotizing gingivitis, and healthy controls. PLoS Negl. Trop. Dis. 6, e1556.View Article
  Brook, I. and Frazier, E.H. 2003. Immune response to Fusobacterium nucleatum and Prevotella intermedia in the sputum of patients with acute exacerbation of chronic bronchitis. Chest 124, 832-33.View Article PubMed
  Carvalho, I., Henriques, M., and Carvalho, S. 2013. New strategies to fight bacterial adhesion. Microbial pathogens and strategies for combating them: science, technology and education In Méndez-Vilas, A. (ed.) FORMATEX 170-78.
  Chong, A., Wehrly, T.D., Nair, V., Fischer, E.R., Barker, J.R., Klose, K.E., and Celli, J. 2008. The early phagosomal stage of Francisella tularensis determines optimal phagosomal escape and Francisella pathogenicity island protein expression. Infect. Immun. 76, 5488-499.View Article PubMed Central PubMed
  CLSI. 2007. Methods for antimicrobial susceptibility testing of anaerobic bacteria; approved standard. 7th ed. Document M11-A7. Clinical and Laboratory Standards Institute, Wayne, PA, USA.
  Denny, T.P., Makini, F.W., and Brumbley, S.M. 1988. Characterization of Pseudomonas solanacearum Tn5 mutants deficient in extracellular polysaccharide. Mol. Plant Microbe Interact. 1, 215-23.View Article
  Donlan, R.M. 2002. Biofilms: Microbial life on surfaces. Emerg. Infect. Dis. 8, 881-90.View Article PubMed Central PubMed
  Ehsani, M., Moghadamnia, A., Zahedpasha, S., Maliji, G., Haghanifar, S., Mir, S.M., and Kani, N.M. 2012. The role of prophylactic ibuprofen and N-acetylcysteine on the level of cytokines in periapical exudates and the post-treatment pain. Daru. 20, 30.View Article PubMed Central PubMed
  Falkler, W.A. Jr., Enwonwu, C.O., and Idigbe, E.O. 1999. Microbiological understandings and mysteries of noma (cancrum oris). Oral Dis. 5, 150-55.View Article PubMed
  Fosse, T., Madinier, I., Hannoun, L., Giraud-Morin, C., Hitzig, C., Charbit, Y., and Ourang, S. 2002. High prevalence of cfxA?-lactamase in aminopenicillin-resistant Prevotella strains isolated from periodontal pockets. Oral Microbiol. Immunol. 17, 85-8.View Article PubMed
  Gokcimen, A., Cim, A., Tola, H.T., Bayram, D., Kocak, A., Ozgüner, F., and Ayata, A. 2007. Protective effect of N-acetylcysteine, caffeic acid and vitamin E on doxorubicin hepatotoxicity. Hum. Exp. Toxicol. 26, 519-25.View Article PubMed
  Gomes, B.P., Drucker, D.B., and Lilley, J.D. 1994. Associations of specific bacteria with some endodontic signs and symptoms. Int. Endod. J. 27, 291-98.View Article PubMed
  Gomes, B.P., Lilley, J.D., and Drucker, D.B. 1996. Associations of endodontic symptoms and signs with particular combinations of specific bacteria. Int. Endod. J. 29, 69-5.View Article PubMed
  Goswami, M. and Jawali, N. 2007. Glutathione-mediated augmentation of ?-lactam antibacterial activity against Escherichia coli. J. Antimicrob. Chemother. 60, 184-85.View Article PubMed
  Goswami, M. and Jawali, N. 2010. N-acetylcysteine-mediated modulation of bacterial antibiotic susceptibility. Antimicrob. Agents Chemother. 54, 3529-530.View Article PubMed Central PubMed
  Goswami, M., Mangoli, S.H., and Jawali, N. 2006. Involvement of reactive oxygen species in the action of ciprofloxacin against Esche
• 作者单位：Ji-Hoi Moon (1) (2)
  Eun-Young Jang (1) (3)
  Kyu Sang Shim (3) (4)
  Jin-Yong Lee (1)
  
  1. Department of Maxillofacial Biomedical Engineering, School of Dentistry, and Institute of Oral Biology, Kyung Hee University, Seoul, 130-701, Korea
  2. Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, 130-701, Korea
  3. Department of Dentistry, Graduate School, Kyung Hee University, Seoul, 130-701, Korea
  4. Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea
  
• 刊物主题：Microbiology;
• 出版者：Springer Netherlands
• ISSN：1976-3794

文摘

N-acetyl cysteine (NAC) is an antioxidant that possesses anti-inflammatory activities in tissues. In the field of dentistry, NAC was demonstrated to prevent the expression of LPS-induced inflammatory mediators in phagocytic cells and gingival fibroblasts during the inflammatory process, but the effect of NAC on oral pathogens has been rarely studied. Here, we examined the effect of NAC against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. NAC showed antibacterial activity against the planktonic P. intermedia with MIC value of 3 mg/ml and significantly decreased biofilm formation by the bacterium even at sub MIC. NAC did not affect the antibiotic susceptibility of planktonic P. intermedia, showing indifference (fractional inhibitory concentration index of 0.5-) results against the bacterium in combination with ampicillin, ciprofloxacin, tetracycline or metronidazole. On the other hand, viability of the pre-established bacterial biofilm exposed to the antibiotics except metronidazole was increased in the presence of NAC. Collectively, NAC may be used for prevention of the biofilm formation by P. intermedia rather than eradication of the pre-established bacterial biofilm. Further studies are required to explore antibacterial and anti-biofilm activity of NAC against mixed population of oral bacteria and its modulatory effect on antibiotics used for oral infectious diseases.