Real-time imaging of glioblastoma using bioluminescence in a U-87 MG xenograft model mouse

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• 作者：Woong Kim (1) (2)
  Bo Ram Kang (1) (2)
  Hye Yun Kim (1) (2)
  Soo Min Cho (1) (2)
  Yong-Deok Lee (3)
  Sehoon Kim (3)
  Jung Young Kim (4)
  Dong Jin Kim (1) (2)
  YoungSoo Kim (1) (2)
  
  1. Center for Neuro-Medicine ; Brain Science Institute ; Korea Institute of Science and Technology ; Hwarangno 14-gil 5 ; Seongbuk-gu ; Seoul ; 136-791 ; Republic of Korea
  2. Biological Chemistry Program ; Korea University of Science and Technology ; 217 Gajungro Yuseong-gu ; Daejeon ; 305-350 ; Republic of Korea
  3. Center for Theragnosis ; Biomedical Research Institute ; Korea Institute of Science and Technology ; Hwarangno 14-gil 5 ; Seongbuk-gu ; Seoul ; 136-791 ; Republic of Korea
  4. Molecular Imaging Research Center ; Korea Institute of Radiological & Medical Sciences ; 75 Nowon-gil ; Gongneung-dong ; Nowon-gu ; Seoul ; 139-706 ; Republic of Korea
  
• 关键词：Bioluminescence imaging ; Glioblastoma ; U ; 87 MG ; Xenograft
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：58
• 期：2
• 页码：243-248
• 全文大小：629 KB
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• 刊物主题：Applied Microbiology; Bioorganic Chemistry; Biological Techniques;
• 出版者：Springer Netherlands
• ISSN：2234-344X

文摘

Glioblastoma multiforme (GBM), the most common malignant brain tumor, is characterized by aggressive proliferation and invasive potential. Xenograft animal models of GBM have critically contributed to evaluation of novel therapeutic agents, drug delivery system, and diagnostic tools. To mimic intrinsic behavior of GBM, orthotopic transplantation of cancer cells and continuous observation of cell growth should be conducted in animal study. Here, we generated xenograft model mouse of GBM in which U-87 MG human glioblastoma cells were intracranially implanted for live imaging. Introducing luciferase gene into U-87 MG cell line enabled real-time observation and quantification of tumor survival and propagation by detecting photon emission derived from luciferase. Our GBM model mouse has potentials to bring great advantages in pharmacological and mechanistic investigation on brain tumors.