Direct determination of the binding sites of cisplatin on insulin-like growth factor-1 by top-down mass spectrometry

详细信息    查看全文

• 作者：Ningbo Zhang (1) (2)
  Huan Liu (1) (2)
  Meng Cui (1)
  Yonggang Du (1) (2)
  Zhiqiang Liu (1)
  Shuying Liu (1)
  
• 关键词：Insulin ; like growth factor ; 1 ; Anticancer drug ; Top鈥揹own ; Mass spectrometry
• 刊名：Journal of Biological Inorganic Chemistry
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：20
• 期：1
• 页码：1-10
• 全文大小：566 KB
• 参考文献：1. Timerbaev AR, Hartinger CG, Aleksenko SS, Keppler BK (2006) Chem Rev 106:2224鈥?248 CrossRef
  2. Casini A, Reedijk J (2012) Chem Sci 3:3135鈥?144 CrossRef
  3. Hu W, Luo Q, Wu K, Li X, Wang F, Chen Y, Ma X, Wang J, Liu J, Xiong S, Sadler PJ (2011) Chem Commun 47:6006鈥?008 CrossRef
  4. Moreno-Gordaliza E, Ca帽as B, Palacios MA, G贸mez-G贸mez MM (2009) Anal Chem 81:3507鈥?516 CrossRef
  5. Zhang N, Du Y, Cui M, Xing J, Liu Z, Liu S (2012) Anal Chem 84:6206鈥?212 CrossRef
  6. Li H, Lin TY, Van Orden SL, Zhao Y, Barrow MP, Pizarro AM, Qi Y, Sadler PJ, O鈥機onnor PB (2011) Anal Chem 83:9507鈥?515 CrossRef
  7. Zhao T, King FL (2010) J Biol Inorg Chem 104:186鈥?92
  8. Zhao T, King FL (2011) J Biol Inorg Chem 16:633鈥?39 CrossRef
  9. Sze CM, Shi Z, Khairallah GN, Feketeova L, O鈥橦air RAJ, Xiao Z, Donnelly PS, Wedd AG (2013) Metallomics 5:946鈥?54 CrossRef
  10. Moreno-Gordaliza E, Ca帽as B, Palacios MA, G贸mez-G贸mez MM (2012) Talanta 88:599鈥?08 CrossRef
  11. Zhang N, Du Y, Cui M, Liu Z, Liu S (2014) Anal Bioanal Chem 406:3537鈥?549 CrossRef
  12. Shaw JB, Li W, Holden DD, Zhang Y, Griep-Raming J, Fellers RT, Early BP, Thomas PM, Kelleher NL, Brodbelt JS (2013) J Am Chem Soc 135:12646鈥?2651 CrossRef
  13. Scotcher J, Bythell BJ, Marshall AG (2013) Anal Chem 85:9164鈥?172 CrossRef
  14. Hartinger CG, Tsybin YO, Fuchser J, Dyson PJ (2008) Inorg Chem 47:17鈥?9 CrossRef
  15. Tran D, Bergholz J, Zhang H, He H, Wang Y, Zhang Y, Li Q, Kirkland JL, Xiao Z-X (2014) Aging Cell 13:669鈥?78 CrossRef
  16. Zhi X, Lamperska K, Golusinski P, Schork NJ, Luczewski L, Golusinski W, Masternak MM (2014) Growth Horm IGF Res Off J Growth Horm Res Soc Int IGF Res Soc 24:137鈥?41 CrossRef
  17. Wolf ED, Gill R, Geddes S, Pitts J, Wollmer A, Gr枚tzinger J (1996) Protein Sci 5:2193鈥?202 CrossRef
  18. Clemmons D (2007) Pituitary 10:121鈥?28 CrossRef
  19. Jeon JH, Kim SK, Kim HJ, Chang J, Ahn CM, Chang YS (2008) Cancer Lett 272:232鈥?41 CrossRef
  20. Sun Y, Zheng S, Torossian A, Speirs CK, Schleicher S, Giacalone NJ, Carbone DP, Zhao Z, Lu B (2012) Int J Radiat Oncol Biol Phys 82:e563鈥揺572 CrossRef
  21. Eckstein N, Servan K, Hildebrandt B, P枚litz A, von Jonqui猫res G, Wolf-K眉mmeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer H-D (2009) Cancer Res 69:2996鈥?003
  22. Matczuk M, Przadka M, Aleksenko SS, Czarnocki Z, Pawlak K, Timerbaev AR, Jarosz M (2014) Metallomics 6:147鈥?53 CrossRef
  23. Lau JK-C, Deubel DV (2005) Chem Eur J 11:2849鈥?855 CrossRef
  24. Heudi O, Cailleux A, Allain P (1998) J Inorg Biochem 71:61鈥?9 CrossRef
  25. Moreno-Gordaliza E, Ca帽as B, Palacios MA, G贸mez-G贸mez MM (2010) Analyst 135:1288鈥?298 CrossRef
  26. Jansson M, Andersson G, Uhl茅n M, Nilsson B, K枚rdel J (1998) J Biol Chem 273:24701鈥?4707 CrossRef
  
• 作者单位：Ningbo Zhang (1) (2)
  Huan Liu (1) (2)
  Meng Cui (1)
  Yonggang Du (1) (2)
  Zhiqiang Liu (1)
  Shuying Liu (1)
  
  1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, 130022, People鈥檚 Republic of China
  2. University of the Chinese Academy of Sciences, Beijing, 100049, People鈥檚 Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Microbiology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1327

文摘

Cisplatin has been widely used in the chemotherapy of a variety of tumors, and the interactions of cisplatin with proteins play very important roles in its side effects and drug resistance, as well as its pharmacokinetics and the biodistribution. Insulin-like growth factor-1 (IGF-1) was found to be associated with the drug resistance of cisplatin. Here, the interaction between cisplatin and IGF-1 was investigated using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. IGF-1-Pt(NH3)Cl was the main mono-adduct and the trans labilization was important to the reaction between IGF-1 and cisplatin, while another special mono-adduct IGF-1-Pt(NH3)Cl2 was observed. The rapid and sensitive top-down mass spectrometry-based approach in linear ion trap mass spectrometer has been developed to identify the binding sites of cisplatin in IGF-1 directly without tedious enzyme digestion. Three binding sites (Met59, Arg56 and Cys6) of cisplatin in IGF-1 were determined. The results not only provide a rapid and efficient way to identify the platinum binding sites in proteins, but also indicate that the binding of cisplatin could promote the fragmentation of IGF-1 and the rupture of disulfide bond.