The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat

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• 作者：Yonghua Han ; Ann Blechl ; Daowen Wang
• 关键词：FISH ; Transgene distribution ; Particle bombardment ; mediated co ; transformation ; Transgenic wheat
• 刊名：Transgenic Research
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：24
• 期：6
• 页码：1055-1063
• 全文大小：1,433 KB
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• 作者单位：Yonghua Han (1) (2)
  Ann Blechl (3)
  Daowen Wang (2)
  
  1. School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China
  2. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China
  3. Western Regional Research Center, USDA-ARS Crop Improvement and Genetics, Albany, CA, 94710, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Molecular Medicine
  Plant Genetics and Genomics
  Animal Genetics and Genomics
  Plant Sciences
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-9368

文摘

Although particle bombardment is the predominant method of foreign DNA direct transfer, whether transgene is integrated randomly into the genome has not been determined. In this study, we identified the distribution of transgene loci in 45 transgenic wheat (Triticum aestivum L.) lines containing co-transformed high molecular weight glutenin subunit genes and the selectable marker bar using fluorescence in situ hybridization. Transgene loci were shown to distribute unevenly throughout the genome and incorporate into different locations along individual chromosomes. There was only a slight tendency towards the localization of transgenes in distal chromosome regions. High proportions of transgenes in separate plasmids integrated at the same site and only 7 lines had 2 or 3 loci. Such loci may not segregate frequently in subsequent generations so it is difficult to remove selectable markers from transgenic lines after regeneration. We also found that three transgene lines were associated with rearranged chromosomes, suggesting a the close relationship between particle bombardment-mediated transgene integration and chromosomal rearrangements. Keywords FISH Transgene distribution Particle bombardment-mediated co-transformation Transgenic wheat