Isolation and characterization of 12 novel microsatellite loci in Hume’s Pheasant, Syrmaticus humiae

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• 作者：Yongjian Bei (1) (2)
  Jinhua Li (1) (3)
  Shaoquan Meng (2)
  Guifen Li (2)
  Binghua Sun (1)
  
• 关键词：Syrmaticus humiae ; Microsatellites loci ; Polymorphism ; Population genetics
• 刊名：Conservation Genetics Resources
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：5
• 期：1
• 页码：19-21
• 全文大小：162KB
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• 作者单位：Yongjian Bei (1) (2)
  Jinhua Li (1) (3)
  Shaoquan Meng (2)
  Guifen Li (2)
  Binghua Sun (1)
  
  1. School of Resource and Environmental Engineering, Anhui University, 111 Jiulong Road, Hefei, 230601, China
  2. College of Life Science and Technology, Yulin Normal University, Yulin, 537000, China
  3. School of Life Science, Anhui Normal University, Wuhu, 241000, China
  

文摘

Hume’s Pheasant, Syrmaticus humiae is a rare and endangered species of pheasants. As a result of habitat loss, poaching and human disturbance, its wild populations have decreased dramatically, and it was listed as a globally near-threatened species by BirdLife/IUCN. Here, we describe the first set of twelve polymorphic microsatellite markers isolated from S. humiae genomic libraries using an enrichment protocol which is able to enrich AC and AG motifs. All the loci were screened from a group of 16 individuals sampled from Guangxi province, and each locus showed a relatively high polymorphism (ranged from 4 to 13 alleles). The observed and expected heterozygosity values are ranged from 0.2 to 0.938 and from 0.303 to 0.925, respectively. Four loci showed deviation from Hardy–Weinberg equilibrium and no linkage disequilibrium was found between pairs of loci. These informative microsatellite markers will be a useful molecular tool for further studies of the population genetic structure and evolutionary history of S. humiae.