Recombinant Lactococcus lactis expressing porcine insulin-like growth factor I ameliorates DSS-induced colitis in mice

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• 作者：Shujie Liu ; Yongming Li ; Bo Deng ; Ziwei Xu
• 关键词：Insulin ; like growth factor I ; Lactococcus lactis ; Colitis ; Intestinal repair
• 刊名：BMC Biotechnology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：2,695 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Life Sciences
  Plant Breeding/Biotechnology
  Stem Cells
  Transgenics
  
• 出版者：BioMed Central
• ISSN：1472-6750

文摘

Background Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. However, the low natural abundance of IGF-I limits its research opportunities and practical application in the fields of medicine and animal husbandry. In this study, a tandem repeat strategy was used to express three copies of the same pIGF-I₃ protein in L. lactis. The activity of recombinant pIGF-I₃ (rpIGF-I₃) was further examined by a mouse model of dextran sulfate sodium (DSS)–induced colitis. In addition, the potential of recombinant L. lactis expressing pIGF-I₃ to reduce inflammatory disease was evaluated.