Black carp growth hormone gene transgenic allotetraploid hybrids of Carassius auratus red var. (♀)×Cyprinus carpio (?

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• 作者：Hao Feng (1)
  YongMing Fu (1)
  Jian Luo (1)
  Hui Wu (1)
  Yun Liu (1)
  ShaoJun Liu (1)
  
• 关键词：transgene ; allotetraploid fish ; growth hormone ; black carp ; ecological safety
• 刊名：Science China Life Sciences
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：54
• 期：9
• 页码：822-827
• 全文大小：808KB
• 参考文献：1. Zhu Z, Li G, He L, / et al. Novel gene transfer into fertilized eggs of goldfish ( / Carassius auratus). J Appl Ichthyol, 1985, 1: 31-4 CrossRef
  2. Zeng Z Q, Zhu Z Y. Transgenes in F4 pMThGH-transgenic common carp ( / Cyprinus carpio L.) are highly polymorphic. Chin Sci Bull, 2001, 46: 143-48 CrossRef
  3. Cachot J, Law M, Pottier D, / et al. Characterization of toxic effects of sediment-associated organic pollutants using the λ transgenic Medaka. Environ Sci Technol, 2007, 41: 7830-836 CrossRef
  4. Fu C, Li D, Hu W, / et al. Fast-growing transgenic common carp mounting compensatory growth. J Fish Biol, 2007, 71: 174-85 CrossRef
  5. Rosa C E, Fiqueiredo M A, Lanes C F, / et al. Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish. Comp Biochem Physiol B Biochem Mol Biol, 2008, 149: 209-14 CrossRef
  6. Abad Z, Gonzalez R, Mendoza I, / et al. Production of a high percentage of male offspring in growth-enhanced transgenic tilapia using / Oreochromis aureus ZZ selected pseudofemales. Aquaculture, 2007, 270: 1- CrossRef
  7. Zhu Z Y, Zeng Z Q. Open a door for transgenic fish to market. Biotech inf, 2000, 1: 1-
  8. Reynold M, Maria T H, Amilcar A, / et al. Tilapia chromosomal growth hormone gene expression accelerates growth in transgenic zebrafish ( / Danio rerio). Elctron J Biotechnol, 2001, 4: 1-
  9. Sun X W, Liang L Q, Yan X C, / et al. Research on transgenic fish as food. High Tech Lett, 1998, 3: 50-5
  10. Wang J L. Transgenic organisms and biosafety. Chin J Ecol, 2006, 25: 314-17
  11. Hu W, Wang Y P, Zhu Z Y. Progress in the evaluation of transgenic fish for possible ecological risk and its containment strategies. Sci China Ser C-Life Sci, 2007, 50: 573-79 CrossRef
  12. Sundstr?m L F, L?hmus M, Johnsson J I, / et al. Growth hormone transgenic coho salmon pay for growth potential with increased predation mortality. Proc R Soc Lond B, 2004, 271: 350-52 CrossRef
  13. Li D L, Fu C Z, Hu W, / et al. Rapid growth cost in “all-fish-growth hormone gene transgenic carp: reduced critical swimming speed. Chin Sci Bull, 2007, 52: 1501-506 CrossRef
  14. Sundstr?m L F, L?hmus M, Devlin R H. Migration and growth potential of coho salmon smolts: implications for ecological impacts from growth-enhanced fish. Ecol Appl, 2010, 20: 1372-383 CrossRef
  15. Sundstr?m L F, L?hmus M, Tvmchuk W E, / et al. Gene-environment interactions influence ecological consequences of transgenic animals. Proc Natl Acad Sci USA, 2007, 104: 3889-894 CrossRef
  16. Jhingan E, Devlin R H, Iwama G K. Disease resistance, stress response and effects of triploidy in growth hormone transgenic coho salmon. J Fish Biol, 2003, 63: 806-23 CrossRef
  17. Devlin R H, Sundstr?m L F, Muir W M. Interface of biotechnology and ecology for environmental risk assessments of transgenic fish. Trends Biotechnol, 2006, 24: 89-7 CrossRef
  18. Liu S J, Liu Y, Zhou G J, / et al. The formation of tetraploid stocks of red crucian carp×common carp hybrids as an effect of interspecific hybridization. Aquaculture, 2001, 192: 171-86 CrossRef
  19. Liu S J, Hu F, Zhou G J, / et al. Gonadal structure of triploid crucian carp produced by crossing allotetraploid hybrids of / Carassius auratus red var. (♀) × / Cyprinus carpio (? with Japanese crucian carp ( / Carassius auratus T. ETS). Acta Hydrobiol Sin, 2000, 24: 301-06
  20. Feng H, Chen J, Luo J, / et al. Cloning of Black Carp β-actin gene and primarily detecting the function of its promoter region. Acta Genet Sin, 2006, 33: 133-40 CrossRef
  21. Feng H, Zeng Z Q, Cheng J, / et al. Molecular cloning and sequencing of growth hormone gene for black carp. Acta Laser Biol Sin, 2002, 11: 407-11
  22. Feng H, Zeng Z Q, Liu S J, / et al. Studies of F1 of transgenic allotraploid hybrids of / Carassius auratus red var. (♀) × / Cyprinus carpio (?. Acta Genet Sin, 2002, 29: 434-37
  23. Feng H, Cheng J, Liu Y, / et al. In Vitro expression and antibody preparation of black carp ( / Mylopharyngodon piceus) GH. Hereditas (Beijing), 2005, 27: 729-34
  24. Devlin R H, Biagi C A, Yesaki T Y, / et al. Growth of domesticated transgenic fish. Nature, 2001, 409: 781-82 CrossRef
  25. Wang Y P, Hu W, Wu G, / et al. Genetic analysis of “all-fish-growth hormone gene transferred carp ( / Cyprinus carpio L.) and its F1 generation. Chin Sci Bull, 2001, 46: 1174-177
  
• 作者单位：Hao Feng (1)
  YongMing Fu (1)
  Jian Luo (1)
  Hui Wu (1)
  Yun Liu (1)
  ShaoJun Liu (1)
  
  1. Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China
  

文摘

Ecological safety is a major consideration in the commercialization of transgenic fish. Development of sterile transgenic triploid fish through hybridization of transgenic tetraploid fish and transgenic diploid fish is a feasible way to solve this problem. The “all-fish-transgene, pbcAbcGHc, containing the black carp β-actin gene promoter and the open reading frame (ORF) of the black carp growth hormone (GH) gene was constructed and introduced into fertilized eggs of allotetraploid fish through microinjection. Contrast cultivation results showed that the growth rate of 150 day-old P0 black carp GH gene transgenic allotetraploid fish was much higher than that of controls. Sixty 150 day-old transgenic allotetraploid fish were assayed by PCR for transgene integration and 90% of fish were positive for the transgene. The transgene was detected in 13 of 20 sperm samples from male transgenic allotetraploid fish. RT-PCR detected transcription of the exogenous black carp GH gene in the muscle, liver, kidney and ovaries of the largest transgenic allotetraploid fish. This study has developed P0 black carp GH gene transgenic allotetraploid fish with a highly increased growth rate, which provides a solid foundation for the establishment of a pure line of transgenic allotetraploid fish and for the large scale production of sterile transgenic triploid fish.