The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

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• 作者：Ji Chen ; Qing Luo ; Hairong Bao ; Lanjie Liao ; Yongming Li ; Zuoyan Zhu…
• 关键词：Common carp ; Growth hormone ; Transgene ; Integration site ; Copy number
• 刊名：Chinese Science Bulletin
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：60
• 期：19
• 页码：1654-1660
• 全文大小：1,378 KB
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• 作者单位：Ji Chen (1)
  Qing Luo (1) (2)
  Hairong Bao (1)
  Lanjie Liao (1)
  Yongming Li (1)
  Zuoyan Zhu (1)
  Yaping Wang (1)
  Wei Hu (1)
  
  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China
  2. University of Chinese Academy of Sciences, Beijing, 100049, China
  
• 刊物主题：Science, general; Life Sciences, general; Physics, general; Chemistry/Food Science, general; Earth Sciences, general; Engineering, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-9541

文摘

The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an “all-fish-growth hormone (GH) transgenic common carp family that exhibits fast growth. In this present study, genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an AT-rich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site. Keywords Common carp Growth hormone Transgene Integration site Copy number