Development and characterisation of EST-SSR markers by transcriptome sequencing in taro (Colocasia esculenta (L.) Schoot)

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• 作者：Yongning You ; Duchen Liu ; Huabo Liu ; Xingfei Zheng ; Ying Diao…
• 关键词：Taro ; Simple sequence repeat marker ; Transcriptome ; Genetic diversity
• 刊名：Molecular Breeding
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：35
• 期：6
• 全文大小：699 KB
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• 作者单位：Yongning You (1)
  Duchen Liu (2)
  Huabo Liu (3)
  Xingfei Zheng (1)
  Ying Diao (1)
  Xinfang Huang (4)
  Zhongli Hu (1)
  
  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, No. 299, Ba Yi Road, Wuchang, Wuhan, 430072, Hubei, People鈥檚 Republic of China
  2. Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province, Horticulture Institute, Sichuan Academy of Agricultural Sciences, No. 20, Jingjushi Road, Jinjiang, Chengdu, 610066, Sichuan, People鈥檚 Republic of China
  3. Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, College of Agricultural, Hubei Engineering University, Xiaogan, 432000, Hubei, People鈥檚 Republic of China
  4. Wuhan National Germplasm Repository for Aquatic Vegetables, Wuhan Vegetable Institute of Science, Wuhan, 430065, Hubei, People鈥檚 Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-9788

文摘

Taro (Colocasia esculenta) is an important crop with a long history of cultivation. In this study 5278 SSRs were identified in taro transcriptome data. A total of 2858 primer pairs were designed for marker development. 100 primers were randomly selected and synthesized. Among them, 72 primer pairs were successfully amplified and 62 were polymorphic in taro accessions. The number of alleles ranged from 2 to 14 for each different polymorphic locus and the polymorphism information content valued ranged from 0.01 to 0.82. The phylogenetic tree was also constructed to analyse the genetic diversity in 68 taro accessions. The large number of taro SSR markers developed in the present study will be useful in the researches of genetic diversity, germplasm characterization and molecular breeding etc.