Dietary supplementation with glutamate precursor α-ketoglutarate attenuates lipopolysaccharide-induced liver injury in young pigs

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• 作者：Lei Wang ; Yongqing Hou ; Dan Yi ; Yongtang Li ; Binying Ding ; Huiling Zhu…
• 关键词：α ; Ketoglutarate ; Piglets ; Lipopolysaccharide ; Liver injury
• 刊名：Amino Acids
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：47
• 期：7
• 页码：1309-1318
• 全文大小：727 KB
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• 作者单位：Lei Wang (1)
  Yongqing Hou (1)
  Dan Yi (1)
  Yongtang Li (1)
  Binying Ding (1)
  Huiling Zhu (1)
  Jian Liu (1)
  Hang Xiao (1)
  Guoyao Wu (1) (2)
  
  1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, China
  2. Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Analytical Chemistry
  Biochemical Engineering
  Life Sciences
  Proteomics
  Neurobiology
  
• 出版者：Springer Wien
• ISSN：1438-2199

文摘

There is growing interest in glutamate as a functional amino acid in nutrition and health. This study was conducted to determine whether glutamate precursor α-ketoglutarate (AKG) could alleviate lipopolysaccharide (LPS)-induced liver injury in young pigs. Twenty-four piglets were randomly assigned to the control, LPS, or LPS?+?AKG group. Piglets in the control and LPS groups were fed a basal diet, whereas piglets in the NAC group were fed the basal diet supplemented with 1?% AKG. On days 10, 12, 14, and 16 of the trial, piglets in the LPS and LPS?+?AKG groups received intraperitoneal administration of LPS (80?μg/kg BW), whereas piglets in the control group received the same volume of saline. On day 16 of the trial, blood samples were collected 3?h after LPS or saline injection. Twenty-four hours post-administration of LPS or saline (on day 17 of the trial), piglets were killed to obtain liver for analysis. Dietary AKG supplementation alleviated LPS-induced histomorphological abnormalities and mitigated LPS-induced increases in aspartate aminotransferase (AST) activity and AST/ALT ratio (P?<?0.05). Compared with the LPS group, dietary supplementation with AKG decreased plasma glutamate concentration, while increasing hepatic concentrations of glutamate, glutamine, leucine, asparagine, lysine, alanine, serine, threonine, valine, and phenylalanine (P?<?0.05). LPS challenge dramatically increased concentrations of malondialdehyde and decreased glutathione peroxidase activity in the liver. Additionally, LPS challenge enhanced concentrations of AMP and total protein, as well as RNA/DNA and total protein/DNA ratios, while decreasing hepatic ADP concentrations. These adverse effects of LPS challenge were ameliorated by AKG supplementation. Collectively, dietary AKG supplementation provides a new means to ameliorate LPS-induced liver injury by increasing anti-oxidative capacity and improving energy metabolism in young pigs.